硅基pH荧光纳米颗粒的制备及其光学性能研究

Research on the fabrication of pH fluorescence nanoparticles based on silicon and their optical properties

荧光素钠的分子内运动会受到刚性无机材料的限制,阻塞非辐射弛豫通道,使放射性衰变填充到基态,提高其发光性能。利用stober法制备了pH响应荧光纳米颗粒。红外分析结果表明,荧光素钠与二氧化硅有效复合。探讨了荧光纳米颗粒的紫外光谱和荧光光谱与溶液pH值的响应关系。在pH值为1~3时,430 nm处紫外吸收强度随pH值增加逐渐降低,在pH值为4~11时,424到484 nm间紫外吸光强度随pH值增加逐渐增加,520 nm时线性良好;而在pH值为4~9之间,荧光强度随pH值增加而升高,并具有较好的响应关系。对荧光纳米颗粒分别在pH值为5和10的缓冲溶液中的荧光光谱进行了干扰测试,几乎不受常见离子干扰,具有良好抗干扰能力。

The intramolecular movement of sodium fluorescein is limited by rigid inorganic materials, blocking non-radiative relaxation channels and allowing radioactive decay to fill in the ground state, which improves its luminescent properties. In this work, pH-responsive fluorescent nanoparticles were prepared by stober method. The results of infrared analysis showed that fluorescein sodium was combined effectively with silica. It was discussed that the response of pH value of solution was related with UV spectrum and fluorescence spectrum of fluorescent nanoparticles. When the pH value is at 1-3, the intensity of UV absorption at 430 nm is decreased gradually with the increase of pH value. While the pH value is 4-11, the intensity of UV absorption between 424 nm and 484 nm is improved slowly with the augmentation of pH value, and the linearity is good at 520 nm. The increment of fluorescence intensity is with the enhancing of pH between 4 and 9, demonstrating a good responsive relationship. The fluorescence spectra of the fluorescent nanoparticles in the buffer solution with pH value of 5 and 10 were tested for disturbance. The findings illustrates that the nanoparticles are almost no influence by ordinary ion and have good ability to resist disturbance.