多囊卵巢综合征颗粒细胞氧化应激相关基因及基因-转录因子调控网络

Oxidative stress-related genes and the genes-transcriptional factors network of PCOS granulosa cell

目的多囊卵巢综合征(PCOS)的发生发展与氧化应激密切相关。本研究旨在鉴定PCOS颗粒细胞的氧化应激相关基因及基因-转录因子网络。方法R软件下载来自基因表达数据库(GEO)的GSE34526数据集,与氧化应激相关的基因通过Python软件在基因本体论(GO)数据库获得。对差异表达的氧化应激相关基因进行GO富集分析、京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)信号通路富集分析和蛋白-蛋白相互作用(PPI)网络分析。最后,使用Network Analyst web工具查找关键差异基因的转录因子(TFs)。结果7例PCOS患者和3例健康对照女性共检测到63个氧化应激相关差异基因。GO和KEGG富集分析显示,差异基因与肿瘤坏死因子(TNF)信号通路、叉头框蛋白O(FoxO)信号通路、凋亡和氧化应激等方面有关。PPI结果显示,各基因间存在显著的交互作用。基于35个关键基因(Hub gene)中的前22个,我们发现了14个转录因子。结论我们鉴定出63个潜在的PCOS颗粒细胞氧化应激相关基因,包括35个关键基因和14个转录因子,它们可能通过调节颗粒细胞氧化应激水平在PCOS不同表型中发挥重要作用。

Objective The occurrence and development of polycystic ovary syndrome(PCOS)is closely connected with oxidative stress.This study aimed to identify the oxidative stress-related genes and the genes-transcriptional factors network of PCOS granulosa cell.Methods The GSE34526 coming from gene expression omnibus(GEO)database was downloaded by R software,the genes related to oxidative stress coming from the gene ontology(GO)database by Python.GO enrichment analysis,Kyoto encyclopedia of genes and genomes(KEGG)signaling pathway enrichment analysis and protein-protein interaction(PPI)network were performed for differentially expressed oxidative stress-related genes.Finally,Network Analyst web tool was used to find the hub genes’transcriptional factors(TFs).Results A total of 63 genes related to differentially expressed oxidative stress were identified in 7 PCOS patients and 3 healthy controls.GO and KEGG enrichment analyses showed that they are related to tumor necrosis factor(TNF)signaling pathway,forkhead box protein O(FoxO)signaling pathway,apoptosis and oxidative stress aspects.PPI results showed that there were significant interactions among genes.According to the top 22 of the 35 Hub genes,we found 14 transcription factors.Conclusion We identified 63 potential oxidative stress-related genes of PCOS granulosa cell,including 35 Hub genes and 14 TFs,which may play an important role in different phenotypes of PCOS by regulating the level of oxidative stress.