妊娠糖尿病患者血清miR-330-3p、lncRNA SNHG17表达水平及临床意义

The expression level and their clinical significance of miR-330-3p and lncRNA SNHG17 in the serun of patients with gestational diabetes mellitus

目的 探究妊娠糖尿病(GDM)患者血清miR-330-3p、长链非编码RNA(lncRNA)小核仁RNA宿主基因17(SNHG17)表达水平及对不良妊娠结局的预测价值。方法 招募2021年1月至2022年2月西北妇女儿童医院产科产检及分娩的160例GDM孕妇(GDM组)和160例糖耐量试验正常的健康孕妇(对照组)为研究对象。荧光定量PCR反应检测血清miR-330-3p、lncRNA SNHG17表达水平;Pearson法分析lncRNA SNHG17与miR-330-3p及二者与空腹血糖(FBG)等生化指标的相关性;多因素Logistic回归分析GDM孕妇妊娠结局的影响因素;受试者操作特征(ROC)曲线分析lncRNASNHG17、miR-330-3p对GDM孕妇不良妊娠结局的预测价值。结果 与对照组相比,GDM孕妇血清糖化血红蛋白(HbA1c)、空腹血糖(FBG)、OGTT后2 h血糖、空腹胰岛素(FINS)、胰岛素抵抗指数(HOMA-IR)、miR-330-3p水平较高(P<0.05),HOMA-β、lncRNA SNHG17较低(P<0.05)。GDM孕妇血清miR-330-3p、lncRNA SNHG17与FBG等生化指标密切相关(P<0.05)。GDM孕妇血清中miR-330-3p与lncRNA SNHG17呈负相关(P<0.05)。Logistic分析显示,在GDM孕妇中,miR-330-3p高水平、lncRNA SNHG17低水平为不良妊娠结局的危险因素(P<0.05)。ROC结果显示,miR-330-3p、lncRNA SNHG17联合对GDM孕妇妊娠结局的预测价值显著优于单独指标(P<0.05)。结论 GDM孕妇血清miR-330-3p水平较高,lncRNA SNHG17较低,二者联合对GDM孕妇不良妊娠结局的预测价值优于单一指标。

Objective To investigate the expression levels of serum miR-330-3p and long non-coding RNA(lncRNA)small nucleolar RNA host gene 17(SNHG17) and the predictive value of adverse pregnancy outcomes in gestational diabetes mellitus(GDM) patients. Methods From January 2021 to February 2022, 160 pregnant women with GDM(GDM group) and 160 healthy pregnant women with normal glucose tolerance(control group) in the Department of Obstetrics and Gynecology,Northwest Women’s and Children’s Hospital were recruited for the study. The expression levels of miR-330-3p and lncRNA SNHG17 in serum were measured by fluorescence quantitative PCR reaction. Pearson correlation analysis of lncRNA SNHG17and miR-330-3p and the correlation between the two and their biochemical indexes such as fasting blood glucose(FBG). Multivariate Logistic regression was used to analyze the influencing factors of pregnancy outcomes in pregnant women with GDM.Receiver operating(ROC) curve was used to analyze the predictive value of lncRNA SNHG17 and miR-330-3p on adverse pregnancy outcomes in pregnant women with GDM. Results Compared with the control group, the levels of serum glycated haemoglobin A1C(HbA1c), fasting blood glucose(FBG), 2 h blood glucose after OGTT, fasting insulin(FINS), homeostasis model assessment of insulin resistance(HOMA-IR) and miR-330-3p were higher in GDM pregnant women(P<0.05), while HOMA-β and lncRNA SNHG17 were lower(P<0.05). Serum miR-330-3p and lncRNA SNHG17 were strongly correlated with biochemical indicators such as FBG in pregnant women with GDM(P<0.05).(P<0.05). Serum miR-330-3p was negatively correlated with lncRNA SNHG17 in pregnant women with GDM(P<0.05). Logistic analysis showed that high level of miR-330-3p and low level of lncRNA SNHG17 were risk factors of adverse pregnancy outcomes in pregnant women with GDM(P<0.05). The ROC results showed that the combined predictive value of miR-330-3p and lncRNA SNHG17 on pregnancy outcome in pregnant women with GDM was significantly better than that of the indicators alone(P<0.05). Conclusion The serum level of miR-330-3p in pregnant women with GDM is elevated, while the level of lncRNA SNHG17 is decreased. The predictive value of the combined two is better than the single indicator for adverse pregnancy outcomes in pregnant women with GDM.