桂花枝、叶HPLC指纹图谱建立及9种成分测定

Establishment of HPLC fingerprints and determination of nine constituents for Osmanthus fragrans branches and leaves

目的建立桂花Osmanthus fragrans L.枝、叶HPLC指纹图谱,并测定红景天苷、特女贞苷、新女贞苷、女贞苷G13、阿魏酸、原儿茶酸、肉桂酸、齐墩果酸、熊果酸的含量。方法2个部位甲醇提取液分析采用YMC-Pack ODS-A色谱柱(250 mm×4.6 mm,5μm);流动相乙腈-0.1%磷酸,梯度洗脱;体积流量1.0 mL/min;柱温30℃;检测波长210 nm。结果20批样品指纹图谱有24个共有峰,相似度大于0.805。9种成分在各自范围内线性关系良好(r≥0.9918),平均加样回收率92.6%~103.5%,RSD 0.99%~2.40%。结论该方法稳定可靠,可用于桂花枝、叶的质量控制。

AIM To establish the HPLC fingerprints for Osmanthus fragrans L.branches and leaves,and to determine the contents of salidroside,protocatechuic acid,neonuezhenide,ferulic acid,spenuezhenide,nuezhenide G13,cinnamic acid,oleanolic acid and ursolic acid.METHODS The analysis of methanol extract of the plant’s branches and leaves was performed on a 30℃thermostatic YMC ODS-A column(250 mm×4.6 mm,5μm),with the mobile phase comprising of acetonitrile-0.1%phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 210 nm.RESULTS The twenty batches of samples were observed with twenty-four common peaks sharing more than 0.805 similarities in the fingerprints.Nine constituents showed good linear relationships within their own ranges(r≥0.9918),whose average recoveries were 92.6%-103.5%with the RSDs of 0.99%-2.40%.CONCLUSION This stable and reliable method can be used for the quality control of O.fragrans branches and leaves.