明胶基抗菌水凝胶的制备与表征

Preparation and Characterization of Gelatin-based Antibacterial Hydrogels

以明胶为基体,N-乙烯基吡咯烷酮(VP)为改性单体,在引发剂作用下通过原位聚合将聚N-乙烯基吡咯烷酮(PVP)分子接枝于明胶分子骨架。然后通过物理吸附和化学键合的方式将碘分子负载到复合水凝胶中,制备明胶基抗菌水凝胶。重点探究了不同单体加入量下PVP的接枝率、相关条件下复合水凝胶的凝胶强度、细胞毒性及碘分子负载量,并对负载碘分子的水凝胶抗菌性进行了分析表征。结果表明:FT-IR测试证实了VP在明胶骨架上发生了接枝反应;VP浓度大小影响接枝分率,在一定条件下VP浓度为0.4 mol/L时接枝分率最高为84.8%;室温下,接枝分率15.2%的PVP导致明胶水凝胶强度降低,但当接枝分率高于50.5%时,凝胶强度增大到与纯明胶相当,与20℃时进行对比分析,说明PVP产生的化学网络对提高高温下的明胶强度很关键;碘分子在PVP/明胶复合体系中虽然总负载变化不大,但化学负载量随接枝分率增加而增加,有利于水凝胶在较高温度下的形态稳定;负载了碘分子的复合水凝胶对金黄色葡萄球菌表现出明显的抑制行为。

Poly(N-vinyl pyrrolidone)(PVP)was grafted onto the framework of gelatin by in situ polymerization using gelatin as substrate and N-vinyl pyrrolidone(VP)as modified monomer.Then through physical adsorption and chemical bonding, iodine molecules were loaded into the composite hydrogel to prepare gelatin-based antibacterial hydrogel.The grafting rate of PVP under different monomer dosage, gel strength, cytotoxicity and iodine molecular load of composite hydrogels under relevant conditions were explored, and the antibacterial properties of hydrogels loaded with iodine molecule were analyzed and characterized.The results showed that the graft reaction of VP on the gelatin skeleton was confirmed by FT-IR test.Under certain conditions, the highest graft rate was 84.8% when the VP concentration was 0.4 mol/L.At room temperature, PVP with grafting rate of 15.2% reduced the gelatine hydrogel strength, but when the grafting rate was higher than 50.5%,the gelatine strength increased to the same as that of pure gelatin.Compared with 20℃,the chemical network generated by PVP was key part to improve the gelatine strength at high temperature.Although the total load of iodine molecule in PVP/gelatin composite system did not change much, the chemical load increased with the increase of grafting rate, which was conducive to the morphology stability of hydrogel at higher temperature.The complex hydrogel loaded with iodine molecule showed obvious inhibition behavior against staphylococcus aureus.