IL-22在复发性流产和不孕妇女子宫内膜着床窗口期的表达和意义

Expression and significance of IL-22 in endometrial implantation window of women with recurrent abortion and infertility

目的 探究IL-22在复发性流产和不孕妇女子宫内膜着床窗口期的表达和意义,为阐明妇女不育不孕相关机制提供科学依据。方法 本研究于2019—2021年在丽水市人民医院纳入不孕妇女(47例)、复发性流产(45例)和健康对照妇女(50例)作为研究对象。在月经周期的着床窗口期进行子宫内膜取样,采用qRT-PCR和免疫组化方法对IL-22的表达情况进行分析。结果 qRT-PCR结果显示,三组子宫内膜IL-22水平比较差异显著,具有统计学意义,(F=23.93,P<0.001)。两两比较结果显示,复发性流产组[(3.41±0.73)μg/L]显著高于不孕组[(2.83±0.68)μg/L]和对照组[(2.46±0.60)μg/L],P均小于0.001,而不孕组显著高于对照组(P=0.006)。免疫组化结果显示,三组子宫内膜腺体和子宫内膜基质IL-22表达差异显著(F=965.38,P<0.001),两两比较结果均显示,复发性流产组>不孕组>对照组(P<0.05)。结论 子宫内膜着床窗口期IL-22表达显著增加,提示其导致妊娠和着床过程中的缺陷,可能与复发性流产相关。复发性流产妇女与不孕妇女IL-22表达差异提示其在这两组妇女中的不同作用。

Objective To explore the expression and significance of IL-22 in the window of endometrial implantation in women with recurrent abortion and infertility, and to provide scientific basis for elucidating the related mechanism of infertility. Methods This study included infertile women(47 cases), recurrent abortion(45 cases) and healthy control women(50cases) in Lishui People’s Hospital from 2019 to 2021. The endometrium was sampled during the implantation window of menstrual cycle, and the expression of IL-22 was analyzed by qRT-PCR and immunohistochemistry. Results The results of qRT-PCR showed that there were significant differences in the levels of IL-22 among the three groups(F=23.93, P<0.001).The results of two comparisons showed that the recurrent abortion group [(3.41±0.73) μg/L] was significantly higher than that of the infertility group [(2.83±0.68) μg/L] and the control group [(2.46±0.60) μg/L], all of which were less than 0.001, while the infertility group was significantly higher than the control group(P=0.006). Immunohistochemical results showed that there were significant differences in the expression of IL-22 in endometrial glands and endometrial matrix among the three groups(F=965.38, P<0.001). The results of pairwise comparison showed that recurrent abortion group > infertility group > control group(P<0.05). Conclusion The expression of IL-22 in endometrial implantation window is significantly increased, which suggests that it leads to pregnancy and implantation defects, which may be related to recurrent abortion. The difference of IL-22 expression between women with recurrent abortion and infertile women suggests its different roles in these two groups of women.