注射用益气复脉(冻干)对力竭游泳大鼠的抗疲劳作用研究

Study on anti-fatigue effect of YiqiFumai Lyophilized Injection on(Carrageenan)exhausted swimming rats

目的:通过大鼠力竭游泳模型考察注射用益气复脉(冻干)(YQFM)的抗疲劳作用。方法:50只Sprague-Dawley(SD)大鼠随机分成对照组、模型组、YQFM低、中、高(231.5、463和926 mg/kg)剂量组,每组10只。检测各组大鼠游泳时间、血清中葡萄糖、乳酸和尿素氮(BUN)及谷胱甘肽过氧化物酶(Gsh-Px)、丙二醛(MDA)、超氧化物歧化酶(SOD)和组织中的肝糖原、肌糖原含量。结果:力竭游泳后大鼠表现出一定的倦态和反应迟钝等现象,YQFM组症状不明显;与模型组相比,YQFM各剂量组大鼠的游泳时间均提高(均P<0.001);与模型组相比,YQFM组葡萄糖、Gsh-Px和MDA、SOD含量和组织中肝糖原、肌糖原水平显著升高(均P<0.001),乳酸、BUN水平显著降低(均P<0.001),均呈剂量依赖性。结论:YQFM可以显著改善力竭游泳大鼠的疲劳症状,补充其体内葡萄糖,减少乳酸堆积和糖原分解,并提高抗氧化活性物质等生化指标水平,对运动性疲劳具有一定的改善作用。

Objective:To investigate the effect of YiqiFumai Lyophilized Injection(YQFM) on anti-fatigue function in rats with exhaustive swimming. Methods:Fifty Sprague-Dawley(SD) rats were randomly divided into control group,exhaustion group and low,middle,and high dose groups(231.5,463,926 mg/kg)of YQFM which 10 in each group. Swimming time,the contents of glucose(Glu),serum lactic acid(Lac),urea nitrogen(BUN),serum glutathione peroxidase(GSH-Px),malondialdehyde(MDA),superoxide dismutase(SOD),liver glycogen and muscle glycogen in tissues were recorded and detected. Results :The exhaustion group showed a certain phenomenon of fatigue and retardation after exhausting swimming,while the symptoms were not obvious in YQFM groups. Compared with the exhaustion group,the swimming time of exhaustive rats increased in each YQFM groups(all P<0.001);Compared with the exhaustion group,the content of Glu,GSH-Px,MDA,SOD and the levels of liver glycogen and muscle glycogen in YQFM groups were significantly increased(all P<0.001),and the levels of Lac and BUN were significantly decreased which the rela tionlac were dose-dependent(all P <0.001). Conclusion:YQFM can significantly improve the state of fatigue,replenish the Glu,reduce Lac accu mulation and glycogenolysis,and increase the biochemical index level of antioxidant activity substancesin exhaustive rats,which has a certain improvement effect caused on exhaustive exercise.