miR-492在舌鳞癌增殖与转移中的作用

The mechanism of miR-492 regulating proliferation,invasion and metastasis of tongue squamous cell carcinoma

目的 阐明miR-492在舌鳞癌发生发展及侵袭转移中的作用及机制,评价其作为舌鳞癌生物标志物及预后评估中的作用,为舌鳞癌的治疗提供新的分子靶点。方法 对52例舌鳞癌及24例正常组织样本分析miR-492的表达差异,分析其表达与临床病理特点之间的关系。通过构建miR-492过表达和低表达舌鳞癌细胞系并检测其细胞增殖、侵袭转移,分析miR-492对舌鳞癌细胞生物学行为的影响。结果 miR-492在舌鳞癌组织中表达高于正常组织(P<0.05),过表达miR-492提高了舌鳞癌细胞的增殖(P<0.05)、转移(P<0.05)、侵袭(P<0.05)能力,抑制miR-492的表达可以抑制舌鳞癌细胞的增殖(P<0.05)、侵袭(P<0.05)、转移(P<0.05)能力。结论 miR-492能促进舌鳞癌细胞的增殖、转移和侵袭,促进舌鳞癌发生发展,可以作为舌鳞癌抗癌新标靶。

Objective To clarify the role and mechanism of miR-492 in the occurrence,development,invasion and metastasis of tongue squamous cell carcinoma,and to evaluate its role as a biomarker of tongue squamous cell carcinoma and its role in the prognosis evaluation of tongue squamous cell carcinoma,this study will provide a new molecular target for the treatment of tongue squamous cell carcinoma.Methods The differences of miR-492 expression in 52 cases of tongue squamous cell carcinoma and 24normal tissue samples were analyzed,and the relationship between miR-492 expression and clinicopathological characteristics was analyzed.The effects of miR-492 on the biological behavior of tongue squamous cell carcinoma cells,including cell proliferation,invasion and metastasis were analyzed by constructing overexpression and under-expression cell lines of miR-492.Results The expression of miR-492 in tongue squamous cell carcinoma tissues was higher than that in normal tissues(P<0.05),and the overexpression of miR-492 increased the ability of proliferation(P<0.05),metastasis(P<0.05) and invasion(P<0.05) of tongue squamous cell carcinoma cells.Inhibition of miR-492 expression could inhibit the proliferation(P<0.05),invasion(P<0.05) and metastasis(P<0.05) of tongue squamous cell carcinoma cells.Conclusion miR-492 can promote the proliferation,metastasis and invasion of tongue squamous cell carcinoma and promote the occurrence and development of tongue squamous cell carcinoma,and can be used as a new anticancer target of tongue squamous cell carcinoma.