生物传感器应用于酱香型酒醅中乳酸、乙醇、葡萄糖的分析研究

Application of Biosensor in the Analysis of Lactic Acid,Ethanol and Glucose in the Fermented Grains of Jiangxiang Baijiu

酱香型酒醅经加水、搅匀、浸泡、过滤、离心,取上清液备用,采用生物传感器分析仪测定葡萄糖、乳酸、乙醇含量。通过准确性、精密度,加标回收试验验证,该方法前处理简单、分析速度快,准确性、精密度和回收率都比较良好。利用该方法对酱香型酒醅1~7轮入池、出池糟进行测定,发现不同轮次的入池酒醅、出池酒醅的葡萄糖、乳酸、乙醇具有一定的差异,入池酒醅的葡萄糖比出池酒醅高,入池酒醅的乙醇比出池酒醅乙醇低,乳酸波动大。

In this study the biosensor is used to detect the content of glucose,lactic acid and ethanol in the fermented grains of Jiangxiang Baijiu.Trough accuracy,precision and spiked recovery tests,the method is proved to be simple and fast,and has good accuracy,precision and recovery.The method is used to detect the pit-entry and pit-exit fermented grains of Jiangxiang Baijiu in the seven rounds of fermentation,and it is found that the content of glucose,lactic acid and ethanol in the fermented grains of different fermentation rounds differs;the glucose content of the pit-entry fermented grains is generally higher than that of the pit-exit fermented grains;the ethanol content of the pit-entry fermented grains is lower than that of the pit-exit fermented grains;the content of lactic acid fluctuates greatly.