西瓜种子EMS诱变技术

EMS mutagenesis technology of watermelon seeds

以甲基磺酸乙酯(EMS)为诱变剂,处理高代自交的西瓜‘97103’种子,设置3个诱变时间(4、8、12 h)和6个诱变浓度(0%、1.5%、2.0%、3.0%、4.0%、5.0%),根据诱变处理后的西瓜发芽率、成苗率来筛选适宜条件组合。结果表明,随着EMS诱变浓度的增大,处理时间的延长,‘97103’的发芽率、成苗率呈下降趋势。为保证一定的存活株数和足量的突变群体,采用发芽率、成苗率接近半致死剂量的条件组合(5.0%EMS溶液处理8 h),作为‘97103’诱变的较理想处理条件。

Three mutagenesis times(4,8,12 h)and six mutagenesis concentrations(0%,1.5%,2.0%,3.0%,4.0%,5.0%)were set to treat high generation self-pollination watermelon‘97103’seeds with ethyl methyl sulfonate(EMS)as mutagenesis agent.The suitable combinations were selected according to the germination rate and seedling rate of watermelon treated with mutagenesis.The results showed that the germination rate and seedling rate of‘97103’showed a decreasing trend with the increase of EMS mutagenic concentration and the increase of treatment time.To ensure a certain number of surviving plants and an adequate mutant community,a combination of conditions with germination and seedling rate close to half lethal dose(5.0%EMS solution treatment for 8 h)was used as a more ideal treatment condition for‘97103’mutagenesis.