鸡肝脏中能量代谢相关膜蛋白基因及其上游转录因子的筛选与鉴定

Identification and screening of energy metabolism-related membrane protein genes and their upstream transcription factors in chicken liver

跨脂质分子层膜蛋白(Tran membrane protein,TMEMs)参与细胞的生理生化过程,对细胞功能的维持具有重要作用。为弄清TMEMs基因在肝脏组织中的表达规律,以不同饲养状态下蛋仔鸡和肉仔鸡的肝脏组织样本,检测8个TMEMs基因的表达情况。结果表明,TMEMs基因在肉鸡肝脏组织中维持较高表达水平,其中TMEM86A基因在肉鸡肝脏中高表达,而在蛋鸡肝脏中低表达,限饲处理会促进TMEM86A基因上调表达;通过对TMEM86A基因启动子区域转录因子预测及启动子活性检测,发现SP1、CREB1可能是TMEM86A的上游转录因子。

Tran membrane proteins(TMEMs)participates in the physiological and biochemical processes of cells and plays an important role in the maintenance of cell function.In order to explore the expression pattern of TMEMs genes in liver tissues,the eight TMEMs genes were chosen to detect their expression levels in liver tissues of broilers and laying hens under the different feeding conditions.The results showed that TMEMs genes maintained a high expression level in the liver tissue of broilers.The expression of TMEM86A gene was high in the liver of broilers and low in the liver of laying hens,and feeding restriction would promote the up-regulated expression of TMEM86A gene.Through the prediction of transcription factors using the promoter region of TMEM86A gene and detection of the promoter activity,it was found that SP1 and CREB1 may be the upstream transcription factors of TMEM86A.