两种培养条件下重组杆状病毒表达蓝舌病病毒VP7蛋白的丰度研究

Study on the abundance of VP7 protein of Bluetongue virus expressed by recombinant Baculovirus under two culture conditions

为了在重组杆状病毒上获得高丰度表达的蓝舌病病毒(BTV)VP7蛋白,利用细胞贴壁培养和摇瓶培养法培养重组杆状病毒,培养5 d,每天采集培养液,用ELISA方法检测其中BTV VP7蛋白的表达量,用SPSS软件对数据进行统计分析。结果显示,贴壁培养条件下BTV VP7蛋白的表达量显著高于摇瓶培养,两种培养条件下BTV VP7蛋白均在第三天时表达量最高,之后开始下降。研究表明用杆状病毒昆虫细胞系统表达BTV VP7蛋白时更适合用贴壁培养方法进行大规模表达。

In order to obtain Bluetongue virus(BTV)VP7 protein with high abundance expression on the recombinant Baculovirus,the recombinant Baculovirus was cultured by cell attachment culture and shake flask culture.After 5 days of culture,the culture medium was collected every day.The expression of BTV VP7 protein was detected by ELISA,and the data were statistically analyzed by SPSS software.The results showed that the expression of BTV VP7 protein in adherent culture was significantly higher than that in shake flask culture.The expression of BTV VP7 protein in both culture conditions was the highest on the third day,and then began to decline.The results showed that the Baculovirus insect cell system was more suitable for large-scale expression of BTV VP7 protein by adherent culture.