仿生石斛多糖对小鼠免疫器官的调节作用

Regulatory Effect of Bionic Dendrobium Polysaccharides on the Development of Immune Organs in Mice

该研究主要通过水提醇沉法提取林下仿生石斛多糖(bionic Dendrobium polysaccharide,BDP),探讨其对免疫抑制小鼠淋巴器官发育的免疫调节机制。利用环磷酰胺(cyclophosphamide,CTX)建立免疫抑制模型,将6周龄C57BL/6J小鼠随机分为模型组、阳性对照组(左旋咪唑组)、仿生石斛多糖组(剂量为120 mg/kg),另设健康小鼠作为空白对照组,各组小鼠灌胃相应的药物10 d,检测免疫脏器系数、观察淋巴结的组织病理学变化,酶联免疫吸附试验(enzyme linked immuno sorbent assay,ELISA)法检测血清中免疫细胞因子白介素2(interleukin-2,IL-2)和白介素6(interleukin-6,IL-6)以及免疫球蛋白G(immunoglobulin G,IgG)和免疫球蛋白M(immunoglobulin M,IgM)。结果表明,BDP可以提高免疫抑制小鼠的体重以及免疫脏器系数,改善环磷酰胺造成的肠系膜淋巴结损伤,提高血清中相关免疫球蛋白IgG和IgM的分泌,增加血清中细胞因子IL-2、IL-6的分泌,维持机体的免疫稳态。

In this study,bionic Dendrobium polysaccharide(BDP) were extracted by water extraction and alcohol precipitation,and the immunomodulatory mechanism on immunosuppressive mice was explored. The immunosuppressive model was induced by cyclophosphamide(CTX)in mice. C57BL/6J mice aged six weeks were randomly divided into a model group,a positive control group(levamisole group),and a BDP group(120 mg/kg),and healthy mice were assigned into the blank control group. Mice in each group were treated with the corresponding drugs by gavage for 10 d. The immune organ index was detected and the histopathological changes in lymph nodes were observed. The serum immune cytokines(IL-2 and IL-6)and immunoglobulins(IgG and IgM)were detected by enzyme linked immuno sorbent assay(ELISA). The results showed that BDP could increase the body weight and immune organ index of immunosuppressive mice,improve the mesenteric lymph node injury caused by CTX,increase the secretion of related immunoglobulins IgG and IgM in serum,promote the secretion of cytokines IL-2 and IL-6 in serum,and maintain the immune homeostasis of the body.