miR-612靶向调控FOXP1对前列腺癌细胞增殖、迁移及侵袭能力的影响

Effects of miR-612 targeting regulation of FOXP1 on proliferation,migration and invasion of prostate cancer cells

目的探讨微小RNA-612(miR-612)对前列腺癌细胞增殖、迁移及侵袭能力的影响及分子机制。方法选择2020年6月至2021年6月在我院住院期间确诊为前列腺腺泡腺癌患者18例,收集标本通过实时荧光定量PCR(RT-qPCR)检测癌组织和癌旁组织中miR-612的表达情况;RT-qPCR检测多种前列腺癌细胞系中miR-612表达;双荧光素酶报告实验检测miR-612与靶基因叉头框蛋白P1(FOXP1)的关系;蛋白印迹检测前列腺癌细胞中FOXP1蛋白表达;MTT法、划痕试验、Transwell检测miR-612过表达和(或)FOXP1过表达对增殖、迁移和侵袭的影响。结果前列腺癌组织和细胞中miR-612的表达显着降低;miR-612过表达显著抑制前列腺癌PC3细胞增殖、迁移和侵袭能力;双荧光素酶报告实验及蛋白印迹检测发现miR-612可靶向负性调控FOXP1的表达,进一步研究发现FOXP1过表达可促进前列腺癌PC3细胞增殖、迁移及侵袭能力;FOXP1过表达逆转了miR-612介导的对前列腺癌PC3细胞增殖、迁移和侵袭的抑制。结论miR-612通过靶向FOXP1部分抑制前列腺癌PC3细胞的增殖、迁移和侵袭能力,miR-612/FOXP1轴可能成为前列腺癌的潜在治疗靶点。

Objective To investigate the effect of microRNA-612(miR-612)on the proliferation,migration and invasion of prostate cancer cells and its molecular mechanism.Methods 18 patients with prostate cancer diagnosed in our hospital from June 2020 to June 2021 were collected to detect the expression of miR-612 in cancer tissues and adjacent tissues by real-time fluorescence quantitative PCR(RT-qPCR),and the expression of miR-612 in prostate cancer cell lines was detected by RT-qPCR.Double luciferase reporter assay was used to detect the relationship between miR-612 and target gene fork box protein P1(FOXP1).Western blotting was used to detect the expression of FOXP1 protein in prostate cancer cell line PC3.MTT,scratch test and Transwell were used to de tect the effects of miR-612 overexpression and/or FOXP1 overexpression on proliferation,migration and invasion.Results The ex pression of miR-612 in prostate cancer tissues andc ell line PC3 was significantly decreased.MiR-612 overexpression significantly inhibited the proliferation,migration and invasion of prostate cancer cells.Double luciferase report assay and Western blotting showed that miR-612 could target and negatively regulate the expression of FOXP1,further studies found that FOXP1 overexpression could promote the proliferation,migration and invasion of prostate cancer cell line PC3.FOXP1 overexpression reversed miR-612-mediated inhibition of proliferation,migration and invasion of prostate cancer cells.Conclusion MiR-612/FOXP1 axis may be a potential therapeutic target for prostate cancer by targeting FOXP1 to partially inhibit the proliferation,migration and invasion of prostate cancer cell line PC3.