甲状腺乳头状癌组织中galecti-3与miR-375水平表达意义及相关性研究

Expression Significance and Correlation of galectin-3 and miR-375 in Papillary Thyroid Carcinoma Tissue

目的探索甲状腺乳头状癌组织中半乳糖凝集素(galectin)-3与微小RNA(micro RNA,miRNA)-375水平表达意义及相关性。方法选取2014年5月~2018年9月于张家口市第一医院普通外科接受手术治疗的87例甲状腺乳头状癌患者作为研究对象,收集其甲状腺癌组织及对应的癌旁组织标本。通过实时荧光定量反应(real time polymerase chain reaction,RT-PCR)检测galectin-3和miR-375在各个组织中的表达情况,分析二者的表达关系及其与临床病理特征的相关性。此外,采用酶联免疫吸附(ELISA)法检测入院时患者血清galectin-3水平,采用RT-PCR法检测血清miR-375水平,通过Kaplan-Meier生存曲线评估血清galectin-3与miR-375表达对甲状腺乳头状癌病人预后的影响。结果与癌旁组织相比,galectin-3在甲状腺乳头状癌中表达上调(1.77±0.48 vs 0.57±0.16),差异有统计学意义(t=22.122,P<0.001);而miR-375在癌组织中的表达水平下调(1.34±0.39 vs 2.12±0.62),差异有统计学意义(t=9.933,P<0.05)。galectin-3表达与肿瘤大小、包膜侵犯、血管浸润、淋巴结转移有关(χ^(2)=9.936~20.028,均P<0.05);miR-375表达与肿瘤大小、血管浸润有关(χ^(2)=12.590,6.403,均P<0.05)。血清galectin-3与miR-375表达水平呈负相关(r=-0.487,P<0.001)。血清galectin-3高表达者三年总体生存期(overall survival,OS)低于低表达者(71.7%vs 93.3%),差异有统计学意义(Log-rank=7.493,P=0.001);miR-375高表达患者的三年OS高于低表达者(93.3%vs 73.9%),差异有统计学意义(Log-rank=7.021,P=0.001)。结论galectin-3在甲状腺乳头状癌组织中表达升高,miR-375在甲状腺乳头状癌组织中表达降低,二者共同参与甲状腺乳头状癌的发生发展,对预后具有一定影响。

Objective To explore the expression significance and correlation of galectin-3 and micro RNA(miR)-375 levels in papillary thyroid carcinoma tissue.Methods 87 patients treated in Department of General Surgery,the First Hospital of Zhangjiakou City from May 2014 to September 2018 were selected as research subjects,and their thyroid cancer tissue and corresponding adjacent cancer tissue specimens were collected.Real-time PCR was performed to investigate the the expression levels of galectin-3 and miR-375 in 87 pairs samples of papillary thyroid carcinoma,and the relationship between galectin-3 and miR-375 expression was analyzed and their relevance to clinicopathologic characteristics of patients with papillary thyroid carcinoma was assessed.Furthermore,serum galectin-3 levels at admission were measured by the enzyme-linked immunosorbent method,and the serum miR-375 levels were measured by RT-PCR.Kaplan-Meier curves were plotted to evlaute the impact of serum galectin-3 and miR-375 expression on the prognosis of papillary thyroid carcinoma patients.Results Compared with adjacent normal tissues,the expression level of galectin-3 was elevated in thyroid cancer tissues(1.77±0.48 vs 0.57±0.16),the difference was statistically significant(t=22.122,P<0.001),but the expression level of miR-375 was reduced(1.34±0.39vs 2.12±0.62),and the difference was statistically significant(t=9.933,P<0.05).Galectin-3 expression was associated with tumor size,capsule invasion,vascular invasion,and lymph node metastasis(χ^(2)=9.936~20.028,all P<0.05).The miR-375expression was related with tumor size and vascular invasion(χ^(2)=12.590,6.403,all P<0.05).There was a significant negative correlation between galectin-3 and miR-375 expression in thyroid carcinoma tissues(r=-0.487,P<0.001).Three-year overall survival(overall survival,OS)was lower in the high serum galectin-3 expression than in the low expression patients(71.7%vs 93.3%),and the difference was statistically significant(Log-rank=7.493,P=0.001),and the 3-year OS of patients with high miR-375 expression was higher than low expression(93.3%vs 73.9%),and the difference was statistically significant(Log-rank=7.021,P=0.001).Conclusion Galectin-3 and miR-375 expression were significantly dysregulated in papillary thyroid carcinoma tissues,and they might be useful biomarkers for the clinical diagnosis and prognostic assessment of papillary thyroid carcinoma.