微生物代谢调控策略研究进展

Advances in Microbial Metabolic Regulation Strategies

微生物正常生长发育过程依赖于细胞内相互交联、配合形成的复杂代谢网络,其中有些通路会产生对人类有益的多种代谢产物,但多数产物生成量少且无法满足高需求,通过修饰改造微生物功能基因可提高目的产物的效率。本文就改变微生物代谢效率所用的代谢工程技术手段,包括微生物通路分析与遗传改造、菌株共培养调控策略、基于计算机预测的新型蛋白质工程以及与生物元件介导的动态调控策略等方面研究进行综述,并提出研究展望,以期为微生物代谢调控相关的研究提供参考。

The process of normal growth and development of microorganisms depends on the complex metabolic network formed by cross-linking and cooperation in cells, some of these pathways may produce a variety of metabolites beneficial to human beings. However, the production of most products is very small,which can not meet the high demand of human beings, and the efficiency of the target products can be improved by modifying the microbial functional genes. In this paper, we discuss metabolic engineering techniques used to change Microbial metabolism efficiency, including microbial pathway analysis and genetic modification, the development of co-culture colony strategies, and new types of protein engineering based on computer prediction, reviewed the aspects of research in the metabolic regulation strategies of biological element-mediated, and put forward the prospect of this research, for further providing reference for the study of microbial metabolism regulation.