水龙骨中没食子酸与鞣花酸的含量测定

Determination of gallic acid and ellagic acid in PolypodiiRhizoma

以Spursil C18色谱柱(250 mm×4.6 mm,5μm)、甲醇-0.1%磷酸水溶液为流动相进行梯度洗脱、流速1.0 mL/min、检测波长255 nm和柱温30℃为色谱条件,建立了HPLC法测定水龙骨中没食子酸与鞣花酸含量的方法。结果表明:没食子酸在浓度为0.5~5.0 mg·L^(-1)的范围内(r=0.9999),鞣花酸在质量浓度为6.944×10^(-3)~69.44×10^(-3)μg的范围内(r=0.9998)与峰面积呈良好的线性关系;没食子酸和鞣花酸平均加样回收率分别为104.21%和101.52%,RSD值分别为4.51%和3.83%;按外标法测得水龙骨中没食子酸含量(n=6)为26.83μg·g^(-1),鞣花酸含量(n=6)为62.18μg·g^(-1)。该测定方法易操作、稳定性及重复性好,为水龙骨药材质量研究提供科学依据。

To establish a HPLC method for the determination of gallic acid and ellagic acid in PolypodiiRhizoma,spursil C18 column(250 mm×4.6 mm,5μm)was used for gradient elution with methanol-0.1%phosphoric acid solution as mobile phase at the flow rate of 1.0 mL/min.The detection wavelength was 255 nm and column temperature was 30℃.It showed that there was a good linear relationship between gallic acid and peak area in the range of 0.5-5.0 mg·L^(-1)(r=0.9999),and there was a good linear relationship between ellagic acid and peak area in the range of 6.944×10^(-3)-69.44×10^(-3)μg(r=0.9998);the average recoveries of gallic acid and ellagic acid were 104.21%and 101.52%,with RSD of 4.51%and 3.83%,respectively.The content of gallic acid(n=6)in PolypodiiRhizoma measured by external standard method was 26.83μg·g^(-1),and the content of ellagic acid(n=6)was 62.18μg·g^(-1).The established method for the determination of gallic acid and ellagic acid is easy to operate,stable and reproducible,which can provide scientific basis for the quality study of PolypodiiRhizoma.