摘要
应用高通量测序技术比较不同预处理对小鼠粪样菌群结构的影响,以期为后续相关研究提供参考依据。采集昆明小鼠新鲜粪样,分为原始粪样组、生理盐水处理组和PBS处理组,提取粪样DNA,采用Illumina MiSeq平台进行测序对3组样本的16S rRNA V3~V4区基因文库进行生物信息学分析。结果显示,3组样本拥有共同的OTUs 188个,不同预处理对粪样微生物多样性和丰度产生影响,生理盐水处理组、PBS处理组和原始粪样组分别检出14、11、12个门,3组样本共有门11个;分别检出24、21、21个纲,3组样本共有纲20个;分别检出62、55、59个科,3组样本共有科26个;分别检出147、126、137个属,3组样本共有属117个。总体来看,生理盐水处理组微生物多样性和丰度相对最高,原始粪样组次之,PBS处理组最低。粪样经生理盐水处理后,能在一定程度上提高肠道菌群检测的准确性。
High-throughput sequencing was used to compare the effect of different pretreatments on faecal flora structure in mice,so as to provide reference for further research.Fresh fecal samples of Kunming mice were collected and divided into three groups:original fecal sample group,saline treatment group and PBS treatment group.Fecal DNA was extracted,sequenced using the Illumina MiSeq platform,and 16S rRNA V3~V4 region gene libraries of three sets of samples were analyzed by bioinformatics analysis.The results showed that there were 188 common OTUs in the three groups,and different pretreatments had an effect on faecal microbial diversity and abundance.In saline treatment group,PBS treatment group and original fecal sample group,14,11 and 12 phyla were detected and there were 11 common phyla in all three groups;24,21 and 21 classes were detected and there were 20 common classes in all three groups;62,55 and 59 families were detected,there were 26 common families in all three groups;147,126 and 137 genus were detected,there were 117 common genus in all three groups.Overall,the saline treatment group had the highest relative microbial diversity and abundance,followed by the original fecal sample group,and the PBS treatment group had the lowest.Saline treatment can improve the accuracy of intestinal flora detection to a certain extent.
作者
刘昕
胡会玲
张敏爱
薛帼珍
闫晓睿
张希春
刘计权
LIU Xin;HU Huiling;ZHANG Minai;XUE Guozhen;YAN Xiaorui;ZHANG Xichun;LIU Jiquan(School of Traditional Chinese Medicine and Food Engineering,Shanxi University of Traditional Chinese Medicine,Shanxi Jinzhong 030600,China;Customs Technical Center of Taiyuan,Taiyuan 030021,China)
出处
《生物技术进展》
2023年第1期115-123,共9页
Current Biotechnology
基金
山西省自然科学基金面上项目(20210302123233)
山西省现代农业产业技术体系建设专项资金(2021-11)
山西中医药大学科技创新科学团队项目(2018TD-009)
山西中医药大学科技创新培育计划项目(2022PY-TH-30)。
关键词
高通量测序
生理盐水
PBS缓冲液
粪样菌群
high-throughput sequencing
saline solution
PBS buffer
fecal microbial communityretreatment