响应面法优化毛大丁草总香豆素提取工艺及其抗氧化作用研究

Optimization of Extraction Process of Total Coumarins from Piloselloides Hirsuta by Response Surface Methodology and its Antioxidant Effect

以毛大丁草为研究对象,选择提取温度、提取溶剂、料液比和提取时间进行单因素试验,在单因素试验基础上,采用Box-Behnken响应面法优化其总香豆素提取工艺,并测定不同产地毛大丁草总香豆素提取率及评价其体外抗氧化活性。结果表明,其最佳提取工艺为提取温度85℃,乙醇体积分数60%,料液比1∶40(g/mL),提取时间60 min。不同产地中清除率最高的为广西百色市样品,IC 50为0.36μg/mL;最低的是贵州黔南州样品,IC 50为121.43μg/mL。不同产地兰香草总黄酮具有一定的总抗氧化能力,能够有效地清除DPPH自由基,效果弱于同浓度条件下V C。经验证该工艺合理、简便,总香豆素提取率可达1.66%。

Single factor experiments were carried out by choosing extraction solvent,solid-liquid ratio,extraction time and extraction temperature from Piloselloides hirsuta.Based on the results of single factor experiments,the Box-Behnken response surface methodology was used to optimize the extraction process of total coumarins,and the contents of total coumarins in Piloselloides hirsuta from different areas was determined and their antioxidant activities in vitro were compared and evaluated.The optimal reflux extraction process were as follows:extraction temperature 85℃,ethanol concentration 60%,solid-liquid ratio 1∶40(g/mL)and extraction time 60 min.The content of total coumarins of Piloselloides hirsuta from ten different areas are varied.Among them,the highest content of total flavonoids is from Baise,Guangxi,with IC 50 of 0.36μg/mL;the lowest content is from Qiannan Prefecture,Guizhou,with IC 50 of 121.43μg/mL.The total coumarins of Piloselloides hirsuta from different areas have a certain total antioxidant capacity,and they can scavenge DPPH radical effectively,which are all weaker than the same concentration of vitamin C.It has been proved that the process is reasonable and simple,and the extraction rate of total coumarins can reach 1.66%.