苦参凝胶对银屑病大鼠皮肤损伤组织病理学及IL-6、PAR-2水平的影响

Effects of Sophora flavescens gel on histopathology, IL-6 and PAR-2 levels in psoriatic rats

目的 研究苦参凝胶对银屑病大鼠皮肤损伤组织病理学及IL-6、蛋白酶活化受体-2(monoclonal antibody to protease activated receptor 2,PAR-2)水平的影响。方法 将60只Wistar大鼠分为对照组、模型组、维A酸组、苦参凝胶高、中、低剂量组,每组10只,观察各组大鼠一般情况、皮肤损伤病理改变、炎症细胞浸润评分、Baker评分及血清和皮肤组织中IL-6、PAR-2水平。结果 维A酸组和苦参凝胶各剂量组大鼠背部皮肤鳞屑、红斑较模型组有所减轻,苦参凝胶组大鼠症状减轻程度优于维A酸组。维A酸组和苦参凝胶各剂量组大鼠背部皮肤病理改变较模型组有不同程度的改善,苦参凝胶中、高剂量组改善程度优于维A酸组。炎症细胞浸润评分、Baker评分及IL-6、PAR-2水平测试,模型组较对照组明显升高(P<0.05),各治疗组较模型组明显降低(P<0.05),苦参凝胶中、高剂量组较维A酸组明显降低(P<0.05)。结论 苦参凝胶对银屑病大鼠皮肤损伤组织病理学表现及IL-6、PAR-2水平具有改善作用。

Objective To study the effects of Kushen gel on histopathology, interleukin-6(IL-6) and protease activated receptor-2(PAR-2) levels in psoriatic rats. Methods Sixty Wistar rats were divided into a control group, a model group, a vitamin A acid group, Kushen gelatin high, medium and low dose groups, 10rats in each group. The general condition, pathological changes of skin lesions, inflammatory cell infiltration score, Baker score and levels of IL-6 and PAR-2 in serum and skin lesions were observed in each group.Results The scales and erythema on the back of rats in each group treated with vitamin A acid and Sophora flavescens gel were alleviated than those in model group. The symptom relief degree of Kushen gel group was better than that of vitamin A acid group. The pathological changes of the skin lesions of rats in different doses of vitamin A acid group and Sophora flavescens gel group were improved to a certain extent than those in model group. The improvement of Kushen gel medium and high dose group was better than that of vitamin A acid group. The inflammatory cell infiltration score, Baker score, IL-6 and PAR-2 levels in the model group were significantly higher than those in the control group(P<0.05). The inflammatory cell infiltration score,Baker score, IL-6 and PAR-2 levels in each treatment group were significantly lower than those in the model group(P<0.05). The inflammatory cell infiltration score, Baker score, IL-6 and PAR-2 levels in the middle and high dose group were significantly lower than those in the A acid group(P<0.05). Conclusions Kushen gel can improve histopathology and IL-6 and PAR-2 levels in psoriatic rats.