肺癌患者癌组织中SHIP2、PLEK2蛋白表达水平及其临床预后意义

Expression Levels of SHIP2 and PLEK2 Proteins in Cancer Tissues of Patients with Lung Cancer and Their Clinical Prognostic Significance

目的分析肺癌患者癌组织中含SH2结构域的Ⅱ型5’肌醇磷酸酶2(SHIP2)、Pleckstrin2(PLEK2)蛋白表达水平及其临床预后意义。方法收集97例肺癌患者的癌组织标本及其癌旁组织(距肿瘤边缘4 cm处)标本。应用免疫组织化学染色法检测所有标本组织中SHIP2、PLEK2蛋白表达水平。采用χ^(2)检验分析肺癌患者癌组织中SHIP2、PLEK2蛋白表达与临床病理特征的关系,多因素Cox回归分析影响肺癌患者预后的相关因素。结果癌组织中SHIP2、PLEK2蛋白高表达率均明显高于癌旁组织(61.86%vs 30.93%,63.92%vs 32.99%;P<0.05)。SHIP2、PLEK2蛋白表达与肺癌患者的TNM分期、淋巴结转移存在显著关系(P<0.05)。SHIP2高表达、低表达组患者3年生存率分别为31.67%(19/60)、59.46%(22/37),PLEK2高表达、低表达组患者3年生存率分别为33.87%(21/62)、57.14%(20/35),SHIP2、PLEK2高表达、低表达组3年生存率比较均存在差异(P<0.05)。单因素分析结果显示:TNMⅢ期、淋巴结转移、SHIP2高表达、PLEK2高表达肺癌患者3年生存时间均明显缩短(P<0.05)。多因素Cox分析结果显示,TNMⅢ期、淋巴结转移、SHIP2高表达、PLEK2高表达均为影响肺癌患者预后的独立危险因素(P<0.05)。结论肺癌患者癌组织中SHIP2、PLEK2蛋白表达均为高表达,并与肺癌TNM分期、淋巴结转移有关,SHIP2高表达、PLEK2高表达的肺癌患者预后较差,二者有望作为评价肺癌患者预后的生物标志物。

Objective To investigate the expression levels of SHIP2 and PLEK2 proteins in cancer tissues of patients with lung cancer and their clinical prognostic significance.Methods The cancerous tissues and their adjacent tissues(4 cm from the tumor edge)of 97 patients with lung cancer were collected.Immunohistochemical staining method was used to detect SHIP2 and PLEK2 protein expression levels in all specimen tissues.The chi-square test was used to analyze the relationship between SHIP2 and PLEK2 protein expression in lung cancer tissues and clinicopathological characteristics.Multivariate Cox regression analysis was used to explore the relevant factors affecting the prognosis of lung cancer patients.Results The high expression rates of SHIP2 and PLEK2 proteins in the cancer tissues were significantly higher than those in the adjacent tissues(61.86%vs 30.93%,63.92%vs 32.99%;P<0.05).SHIP2 and PLEK2 protein expression were significantly correlated with the TNM staging and lymph node metastasis of lung cancer patients Relationship(P<0.05).The 3-year survival rates of patients in the SHIP2 high expression and low expression groups were 31.67%(19/60)and 59.46%(22/37),respectively.The 3-year survival rates of the patients in the PLEK2 high expression and low expression groups were 33.87%(21/62),57.14%(20/35),there were differences in the 3-year survival rate of SHIP2,PLEK2 high expression and low expression groups(P<0.05).The results of univariate analysis showed that the 3-year survival time of lung cancer patients with TNM stageⅢ,lymph node metastasis,SHIP2 high expression,and PLEK2 high expression were significantly shortened(P<0.05).The results of multivariate Cox analysis showed that TNM stageⅢ,lymph node metastasis,high expression of SHIP2,and high expression of PLEK2 were all independent risk factors affecting the prognosis of lung cancer patients(P<0.05).Conclusion The expressions of SHIP2 and PLEK2 proteins in lung cancer tissues are all highly expressed,and both are related to lung cancer TNM staging and lymphatic metastasis.The prognosis of lung cancer patients with high SHIP2 or PLEK2 expression is poor,and both are expected to be used as biomarkers to evaluate the prognosis of lung cancer patients.