基于金纳米颗粒为基底的表面增强拉曼光谱快速鉴别人肝癌细胞与正常肝细胞

Rapid identification of human hepatocellular carcinoma cells and normal liver cells by surface-enhanced Raman spectroscopy based on gold nanoparticles

目的:采用金纳米颗粒为基底的表面增强拉曼光谱(surface-enhanced Raman spectroscopy,SERS)技术对人体正常细胞和不同种类肝癌细胞进行快速、准确地鉴别。方法:将肝癌细胞进行体外培养,金纳米颗粒作为基底加入培养基中进行孵育30 min,通过表面增强拉曼光谱测定人肝癌细胞。结果:基于金纳米颗粒为基底的表面增强拉曼光谱能够快速检测鉴别,呈现肝癌细胞的拉曼光谱,其中包括人肝癌细胞HepG2(RSD 1.20%)、人胆管细胞型肝癌细胞HCCC-9810(RSD 11.14%)、人肝母细胞瘤细胞HuH-6(RSD 9.50%)、人肝癌细胞SK-Hep(RSD 4.60%)、人正常肝细胞HL7702(RSD 4.50%)。并且用其他肿瘤细胞进行了对照,最后对SERS图谱进行峰归属。结论:金纳米颗粒为基底的表面增强拉曼光谱能够对人肝癌细胞进行快速鉴别。

Objective:To perform rapid and accurate identification of normal hepatocytes and different types of hepatocellular carcinoma cells using gold nanoparticle-based surface-enhanced Raman spectroscopy(SERS).Methods:Liver cancer cells were cultured in vitro and gold nanoparticles were added to the culture medium for 30 min.Accordingly,human hepatocellular carcinoma cells were determined using SERS.Results:The results revealed that gold nanoparticle-based SERS could rapidly detect and identify human hepatocellular carcinoma cells,including human hepatocellular carcinoma cells HepG2(RSD 1.20%),hepatic cholangiocarcinoma cells HCCC-9810(RSD 11.14%),human hepatoblastoma cells HuH-6(RSD 9.50%),human hepatocellular carcinoma cells SK-Hep(RSD 4.60%),and human normal hepatocytes HL7702(RSD 4.50%).These cell types were then compared with other tumour cells.Finally,peak assignments were performed on the SERS spectra.Conclusion:This investigation conclude that gold nanoparticle-based SERS is functionally capable of accurate and rapid identification of human hepatocellular carcinoma cells.