摘要
为了探究不同挤压温度(40、60、80、100和120℃)对大豆分离蛋白(Soy Isolate Protein,SPI)与葡萄籽原花青素(Grape Seed Proanthocyanidin Extract,GSPE)复合物功能性质及结构特性的影响。该研究以溶解度、乳化性、乳化稳定性、ζ-电位、粒度为指标,利用荧光光谱、红外光谱分析该复合体系中大豆分离蛋白功能性质及结构的变化。结果表明:相较于挤压SPI,经过挤压处理的SPI-GSPE复合物的溶解度、乳化活性指数、乳化稳定性指数、ζ-电位绝对值及持水性均显著提高(P<0.05),其表面疏水性、持油性显著下降(P<0.05)。随着挤压温度的升高,SPI-GSPE复合物的溶解度、持油性及乳化活性均先增大后减小且在80℃达到最大值,而其表面疏水性先减小后增大且最小值在80℃,ζ-电位绝对值、乳化稳定性及持水性均随温度的升高而降低。粒径分析结果表明,挤压处理后SPI与GSPE形成了更加致密的复合物;荧光光谱及红外光谱结果表明,与GSPE的复合及挤压处理使SPI氨基酸残基所处微环境发生变化,蛋白结构发生变化。以上结果表明挤压温度为80℃时SPI-GSPE复合物功能性质提高幅度最大,为GSPE与SPI复合提高SPI的功能性质提供参考。
The interaction between macromolecules and small molecules has gradually attracted widespread interest in recent years,especially for the interaction between proteins and polyphenols in food components.Phenolic compounds are the parts of the secondary metabolites,mainly found in plant species with great structural diversity.They can exist as glycosides or aglycones,mainly composed of polymeric or monomer structures.Protein is widely found in animals and plants.In the course of food processing,the protein and polyphenols can inevitably coexist in the same system and then interact with each other,which often changes the structure of protein levels.There are some effects of the interaction between polyphenols and proteins on the functional properties of proteins,mainly including the hydration properties(water retention,viscosity,and solubility)and surface properties(foaming and emulsification).The beneficial interactions can improve the nutritional and functional properties of foods.However,the interaction between proteins and polyphenols is susceptible to a variety of factors,including internal factors(self factors,such as the structural characteristics and relative concentrations of proteins and polyphenols),and external factors(environmental factors,mainly including the solvent composition and solution parameters,such as temperature,ionic strength,and p H).The extrusion technique is a physical modification to integrate multiple unit operations,such as material mixing,homogenization,cooking,and molding.In this study,an attempt was made to clarify the effect of proanthocyanidins on the physicochemical properties of soy protein as a representative plant protein,considering the important role of proteins and phenols in health,nutrition,and food quality.Specifically,an investigation was implemented on the effects of different extrusion temperatures(40,60,80,100,and 120℃)on the functional and structural properties of Soybean Protein Isolation(SPI)and Grape Seed Procyanidins Extractive(GSPE)complex.The indicators were selected as emulsifying activity index,emulsion stability index,zeta potential,and particle size.Fluorescence and infrared spectroscopy were used to analyze the functional properties and structure of SPI in the composite system.The results show that there were significantly improved solubility,emulsifying activity,emulsifying stability,absolute value of Zeta potential,and water holding capacity of SPI-GSPE composite after extrusion treatment,compared with the extruded SPI.But,there was an observable decrease in the surface hydrophobicity and oil-holding capacity of the SPI-GSPE composite.Specifically,the solubility,oil retention,and emulsification activity of SPI-GSPE composite first increased,then decreased,and finally reached the maximum at 80℃,with the increase of extrusion temperature.By contrast,the surface hydrophobicity of the SPI-GSPE composite first decreased,then increased,and finally reached the minimum at 80℃.The absolute value of Zeta potential,emulsification stability,and water retention all decreased with the increase in temperature.The particle-size analysis showed that the SPI and GSPE formed a more compact complex after extrusion.The fluorescence spectrum showed that the composite and extrusion with the GSPE were used to extend the skeleton of SPI polypeptide chain for the protein structure,indicating a significant quenching effect on the SPI fluorescence.Ir spectra showed that the non-covalent cross-linking of GSPE to SPI varied greatly in the secondary structure of proteins.The composition and extrusion with the GSPE increased theα-helix,irregular crimp,andβ-corner contents of the SPI,but decreased theβ-fold contents.This finding can provide important theoretical guidance for the utilization of SPI and GSPE resources.
作者
王振国
高育哲
时家峰
张俊杰
江睿生
霍金杰
苏爽
肖志刚
Wang Zhenguo;Gao Yuzhe;Shi Jiafeng;Zhang Junjie;Jiang Ruisheng;Huo Jinjie;Su Shuang;Xiao Zhigang(Grain Science and Technology,College of Shenyang Normal University,Shenyang 110034,China)
出处
《农业工程学报》
EI
CAS
CSCD
北大核心
2022年第21期279-286,共8页
Transactions of the Chinese Society of Agricultural Engineering
基金
辽宁省“揭榜挂帅”科技攻关专项计划(2021JH/104000340201)
国家自然科学基金(32072139)。
关键词
挤压
结构特性
功能特性
原花青素
大豆分离蛋白
extrusion
structural properties
functional properties
procyanidins
soy isolate protein