抗氧化乳酸菌的筛选及其对小肠上皮细胞氧化损伤的保护作用

Screening of Lactic Acid Bacteria with Antioxidant Capacity and Its Protective Effect on Oxidative Damage in Intestinal Epithelial Cells

从36株分离自长寿老人粪便的乳酸菌中筛选高抗氧化活性菌株,利用过氧化氢(H_(2)O_(2))建立大鼠小肠上皮细胞(IEC-6)氧化损伤模型,探究所筛选菌株对IEC-6细胞氧化应激的影响。结果表明,菌株HN-04、HN-05、HN-15有较强的体外抗氧化能力,其DPPH自由基、羟基自由基、超氧阴离子自由基清除能力分别为52.18%~57.16%、47.44%~52.38%、37.11%~43.31%,菌株对IEC-6细胞的黏附率分别为6.77%、6.92%和5.82%。在3株乳酸菌的干预作用下,氧化损伤细胞的存活率提高了5.31%~11.19%,细胞中的谷胱甘肽过氧化物酶(GSHPx)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性和总抗氧化能力(T-AOC)分别提升12.03%~32.39%、21.20%~47.42%、36.10%~87.14%和23.55%~48.69%,丙二醛含量(MDA)降低20.98%~48.80%。所筛选的3株乳酸菌具有缓解细胞氧化应激损伤的作用,可作为天然抗氧化剂应用于功能性食品开发。

Antioxidant strains were screened from 36 lactic acid bacteria isolated from longevity elderly faces,and the oxidative damage model of small intestinal epithelial cells(IEC-6)was established by hydrogen peroxide(H_(2)O_(2))to investigate the effects of the screened strains on the oxidative stress of IEC-6 cells.The results showed that that strains of HN-04,HN-05 and HN-15 had higher antioxidant capacity in vitro,and the scavenging capacity of DPPH radical,hydroxyl radical and superoxide anion radical of the three strains were 52.18%~57.16%,47.44%~52.38%and 37.11%~43.31%.The adhesion rates of the strains to IEC-6 cells were 6.77%,6.92%and 5.82%,respectively.With the intervention effects of the stains,the survival rate of IEC-6 cells was increased by 5.31%~11.19%,and the activities of glutathione peroxidase(GSH-PX),superoxide dismutase(SOD),catalase(CAT)and total antioxidant capacity(T-AOC)in cells were increased by 12.03%~32.39%,21.20%~47.42%,36.10%~87.14%and 23.55%~48.69%,respectively.In addition,the contents of malondialdehyde(MDA)in oxidative damaged IEC-6 cells were decreased by 20.98%~48.80%under the treatment with the strains.In summary,the three strains screened in this research could reduce oxidative stress injury of IEC-6 cells,and could be used as natural antioxidants in the development of functional foods.