基于电子顺磁共振法对枸杞提取液的抗氧化活性研究

Study on Antioxidant Activity of Wolfberry Extract Based on Electron Paramagnetic Resonance Method

运用电子顺磁共振波谱法(EPR),通过单因素和均匀试验研究了枸杞提取液的抗氧化能力(DPPH·清除率)。单因素试验和均匀试验结果表明,在料液比为1∶50、溶剂比(石油醚∶无水乙醇)为1∶500、超声时间为30 min、超声功率为40 W的条件下DPPH·清除率和β-胡萝卜素含量最大。优化条件下枸杞提取液对DPPH·的清除率为92.34%,对羧基自由基的清除率为79.11%,对羟基自由基的清除率为45.54%,β-胡萝卜素含量为0.424 mg/mL,类胡萝卜素含量为141.6 mg/100 g,黄酮含量为0.039 2 mg/mL,枸杞多糖含量为0.066 7 mg/mL。

The antioxidant capacity(DPPH· scavenging rate) of wolfberry extract is studied by single factor test and uniform test using electron paramagnetic resonance method(EPR). Single factor test and uniform test results show that when solid-liquid ratio is 1∶50, solvent ratio(petroleum ether∶absolute ethanol) is 1∶500, ultrasonic time is 30 min, ultrasonic power is 40 W, the scavenging rate of DPPH radical is the highest and the content of β-carotene is the largest.Under the optimized conditions, the scavenging rates of wolfberry extract on DPPH radical, carboxyl radical and hydroxyl radical are 92.34%, 79.11%, 45.54% respectively, the content of β-carotene, carotenoid, flavonoids and wolfberry polysaccharides is 0.424 mg/mL, 141.6 mg/100 g, 0.039 2 mg/mL, 0.066 7 mg/mL respectively.