摘要
目的:基于性状鉴别、浸出物及含量测定研究,评价不同生长年限云南重楼药材质量。方法:采用传统经验鉴别药材外观性状,运用热浸法测定药材醇溶性浸出物含量,采用高效液相色谱法对样品中重楼皂苷Ⅰ、重楼皂苷Ⅱ、重楼皂苷Ⅲ、重楼皂苷Ⅳ、重楼皂苷Ⅴ、重楼皂苷Ⅵ、重楼皂苷Ⅶ、重楼皂苷D和重楼皂苷H的含量进行测定。结果:不同生长年限云南重楼药材外观性状、浸出物、有效成分含量差异较大。随着生长年限的增加,云南重楼药材单个质量、直径及体积明显增加;8年生云南重楼药材醇溶性浸出物质量分数最高;就药材的有效成分含量而言,7年生药材9种重楼皂苷总质量分数最高。结论:综合性状鉴别、浸出物及含量测定结果,最终初步确定云南重楼以7年生药材质量为最佳。
Objective:To evaluate the quality of Paridis Rhizoma in different growth years based on character identification,extraction,and content determination.Methods:The appearance characters of Paridis Rhizoma were identified by traditional experience.The content of alcohol-soluble extracts was determined by the hot dipping method,and the content of polyphyllinsⅠ,Ⅱ,Ⅲ,Ⅳ,Ⅴ,Ⅵ,Ⅶ,D,and H in Paridis Rhizoma samples was determined by highperformance liquid chromatography(HPLC).Results:There were significant differences in the appearance characters,extracts,and content of active components of Paridis Rhizoma in different growing years.With the increase in growth years,the individual weight,diameter,and volume of Paridis Rhizoma increased obviously.The content of alcohol-soluble extracts of 8-year-old Paridis Rhizoma was the highest.In terms of the content of active components in Paridis Rhizoma,the total content of polyphyllinsⅠ,Ⅱ,Ⅲ,Ⅳ,Ⅴ,Ⅵ,Ⅶ,D,and H in 7-year-old Paridis Rhizoma was the highest.Conclusion:Based on the results of character identification,extraction,and content determination,it is preliminarily determined that 7-year-old Paridis Rhizoma has the optimal quality.
作者
王璇
贾天颖
珍桑拉姆
徐文娟
李春帅
杨琳琳
张姝妍
文佳
程水清
王旭星
徐新房
李向日
WANG Xuan;JIA Tian-ying;ZHEN Sang-la-mu;XU Wen-juan;LI Chun-shuai;YANG Lin-lin;ZHANG Shu-yan;WEN Jia;CHENG Shui-qing;WANG Xu-xing;XU Xin-fang;LI Xiang-ri(School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing Key Laboratory for Quality Evaluation of Chinese Materia Medica,Beijing 102488,China;Tongrentang Pharmaceutical Factory of Beijing Tongrentang Co.,Ltd.,Beijing 102629,China)
出处
《中国现代中药》
CAS
2022年第12期2450-2454,共5页
Modern Chinese Medicine
基金
国家中药标准化项目(ZYBZH-Y-YN-44)
国家药典委员会标准制修订研究项目(2018Z006)。
关键词
云南重楼
生长年限
含量测定
质量研究
Paridis Rhizoma
growth years
content determination
quality research