结直肠癌组织CALM1基因表达改变、EGFR突变情况及其临床意义

Changes of CALM1 gene expression and EGFR mutation in colorectal cancer and their clinical significance

目的探讨结直肠癌组织钙调蛋白1(CALM1)基因表达改变和表皮生长因子受体(EGFR)突变情况及其临床意义。方法选择100例结直肠癌患者作为研究对象,均行手术治疗。收集所有患者手术切除癌组织和切缘正常组织标本,检测结直肠癌癌组织和切缘正常组织CALM1基因表达及EGFR突变情况。比较不同临床病理特征患者癌组织CALM1基因表达水平及EGFR突变情况。术后随访3年,观察患者生存情况,运用Kaplan-Meier法对CALM1不同表达水平患者及EGFR突变型与野生型患者进行生存分析。结果结直肠癌组织CALM1基因表达水平及EGFR突变型占比均高于切缘正常组织(t=28.09,χ^(2)=17.36,P均<0.05);有淋巴结转移、Dukes分期C期、低分化程度患者CALM1基因高表达、EGFR突变型比例高于无淋巴结转移、Dukes分期A~B期、高/中分化程度患者(χ^(2)分别=11.79、7.70;7.96、6.18;7.30、8.24,P均<0.05);CALM1基因高表达和EGFR突变型患者3年生存率低于CALM1基因低表达和EGFR野生型患者,差异均有统计学意义(χ^(2)分别=14.85、13.27,P均<0.05)。结论结直肠癌癌组织CALM1基因表达水平及EGFR突变型占比均高于切缘正常组织,二者均与患者临床病理特征和术后生存情况有关。

Objective To investigate the changes of calmodulin 1(CALM1)gene expression and epidermal growth factor receptor(EGFR)mutations in colorectal cancer(CRC)and their clinical significance.Methods A total of 100 patients with CRC were selected as the research subjects,all of whom underwent surgical treatment.The specimens of surgically resected cancer tissues and normal tissues at the cutting edge of all patients were collected,and the expression of CALM1 gene and EGFR mutation in cancer tissues and normal tissues at the cutting edge were detected.The expression level of CALM1 gene and EGFR mutation in cancer tissues of patients with different clinicopathological characteristics were compared.The patients were followed up for 3 years,the survival of patients with different expression levels of CALM1 and EGFR mutant and wild-type patients were analyzed by Kaplan-Meier method.Results The expression level of CALM1 gene and the proportion of EGFR mutant in cancer tissues were higher than those in normal tissues at the cutting edge(t=28.09,χ^(2)=17.36,P<0.05).The high expression of CALM1 gene and the proportions of EGFR mutations in patients with lymph node metastasis,Dukes stage C and low differentiation were higher than those without lymph node metastasis,Dukes stage A-B and high/medium differentiation(χ^(2)=11.79,7.70,7.96,6.18,7.30,8.24,P<0.05).The overall 3-year survival rates of patients with high expression of CALM1 gene and EGFR mutation were statistically lower than the patients with low expression of CALM1 gene and EGFR wild type(χ^(2)=14.85,13.27,P<0.05).Conclusion The expression level of CALM1 gene and the proportion of EGFR mutation in CRC tissues are higher than those in normal tissues,and both of which were related to the clinicopathological characteristics and postoperative survival of patients.