橡胶树炭疽菌孢子的拉曼光谱特征及其在聚类分析中的应用

Raman spectroscopic characteristics of Colletotrichum spores associated with anthranose of rubber tree and their application in cluster analysis

为了弥补分类学上单纯依靠形态学与分子生物学鉴定炭疽菌的不足,基于共聚焦显微拉曼技术的橡胶树炭疽菌3个种群孢子聚类分析方法,对橡胶树炭疽菌孢子的拉曼光谱进行扫描,找到了其共有的位于1005 cm^(-1),1155 cm^(-1)和1515 cm^(-1)处的3个主要的拉曼峰以及其他次级峰,并对峰的产生来源进行初步确认。结合拉曼光谱与主成分分析(PCA),依靠3个主要的拉曼峰以及其他次级峰在PC1、PC2以及PC3的三维空间得分图中快速有效地将3种橡胶树炭疽菌孢子区分开来,为鉴别炭疽菌孢子提供了一种新方法,满足了灵敏与微量的要求,且样品无需预富集和预处理。

In order to solve the shortcomings of traditional identification of Colletotrichum spp.by morphology and molecular biology,a clustering method based on confocal Raman microscopy was proposed for identification of spores of three Colletotrichum species causing anthracnose of rubber trees.Raman spectra of the spores were scanned to show their spectral trends and peak characteristics,and three main Raman peaks at 1005cm^(-1),1155 cm^(-1),and 1515 cm-1in common and other secondary peaks were found to preliminary confirm the contributing source of the peaks.Then,combined with Raman spectroscopy and principal component(PCA)analysis,the spores of three Colletotrichum species were quickly and efficiently distinguished by the three key Raman peaks and other secondary peaks in the 3D score plot of PC1,PC2,and PC3 components.This method provides a new way for discrimination and identification of Colletotrichum spores of the anthracnose of rubber trees,and satisfies the sensitivity and trace requirements without pre-enrichment and pre-treatment of the samples.