海南3种相思树红根病病原菌的鉴定

Identification of pathogenic Ganoderma causing red root rot on three Acacia species in Hainan

为进一步明确引起海南相思树根腐病的病原灵芝菌种类,利用形态学与分子生物学鉴定手段对发生于海南的台湾相思(Acacia. confuse Merr.)、马占相思(A. mangium Willd.)和耳叶相思(A. auriculiformis A.Cunn. ex Benth.)等3种相思树上的红根病的病原灵芝菌进行了种类鉴定。从海南省海口、儋州、昌江等市县采集3种相思树红根病病原菌的担子果,对其进行形态学鉴定。采用组织分离法分离菌株,完成了3种病原菌的致病性测定,构建了基于ITS、SSU与LSU基因的多基因系统发育树,结合形态学与分子生物学鉴定结果,确定这3种相思树红根病病原灵芝菌均为热带灵芝[Ganoderma tropicum (Jungh.) Bres.]。

An attempt was made to identify the pathogenic Ganoderma causing red root rot on Acacia confusa,A. mangium and A. auriculiformis in several areas in Hainan in terms of morphology and molecular biology in order to provide a reference for the control of red root rot on Acacia spp. The basidiocarps of three pathogens causing red root rot of Acacia spp. were collected from Haikou, Danzhou, Changjiang and other cities or counties in Hainan, and their morphological characteristics were observed. The strains were isolated from the three pathogens by tissue isolation method, and their pathogenicity was determined. A polygenic phylogenetic tree was constructed based on genes ITS, SSU and LSU of the strains. The results from the morphological observation and molecular biological identification showed the pathogens causing red root rot on three Acacia species were all identified as Ganoderma tropicum.