基于16S rRNA测序技术分析乳源性复合益生菌对糖尿病小鼠肠道菌群的影响

Analysis of the Effect of Milk Derived Compound Probiotics on Intestinal Flora of Diabetes Mice based on 16S rRNA Sequencing

该研究旨在从肠道菌群角度研究乳源性复合益生菌对db/db糖尿病小鼠的作用机理。将32只db/db糖尿病小鼠分为模型组,阳性药(二甲双胍)组,复合益生菌高、低剂量组,8只db/m鼠作为正常对照组。采用ELISA法测定糖化血红蛋白(glycosylated hemoglobin,type A1C,HbA1c)、血清总胆汁酸(total bile acid,TBA)、血清胰高血糖素样肽1(glucagon-like peptide 1,GLP-1)水平,采用16S rRNA测序技术分析各组小鼠肠道菌群变化情况。结果显示与模型组相比,高、低剂量复合益生菌均能显著降低HbA1c、血清TBA水平,升高血清GLP-1水平;模型组小鼠肠道菌群结构较正常组发生变化,复合益生菌组小鼠肠道菌群结构较模型组发生变化;在菌属水平,模型组Proteobacteria、Enterobacteriaceae等富集,复合益生菌低剂量组norank_f__Lachnospiraceae、Lachnospiraceae_UCG-006、Butyricicoccus、Anaerotruncus等富集,复合益生菌高剂量组Escherichia-Shigella、unclassified_f__Eggerthellaceae、Family_ⅩⅢ_UCG-001富集。复合益生菌可能通过调节糖尿病小鼠肠道菌群失调从而发挥治疗糖尿病的作用,具体机制有待进一步研究。

The purpose of this study was to investigate the mechanism of milk-derived compound probiotics on db/db diabetic mice from the perspective of intestinal flora.Thirty-two db/db diabetic mice were divided into model group,rosiglitazone group,compound probiotics high-dose and low-dose groups,and eight C57BL/KS mice were used as normal control group.HbA1c (glycosylated hemoglobin,type A1C),TBA (total bile acid),GLP-1 (glucagon-like peptide 1) levels were determined by ELISA.16S rRNA sequencing was used to analyze the changes of intestinal flora in each group.The results showed that compared with the model group,both high-dose and low-dose compound probiotics could significantly reduce HbA1c and serum TBA,and increase serum GLP-1.Compared with the normal group,the intestinal microflora structure of mice in the model group was changed,and the structure of intestinal flora in the compound probiotic group was changed compared with that in the model group.At the genus level,Proteobacteria and Enterobacteriaceae in the model group are enriched.In low dose group norank_f__Lachnospiraceae,Lachnospiraceae_UCG-006,Butyricicoccus and Anaerotruncus are enriched.In compound probiotics high dose group,Escherichia-Shigella,Unclassified_f__Eggerthellaceae,and Family_ⅩⅢ_UCG-001 were enriched.Compound probiotics may play a role in the treatment of diabetes by regulating intestinal microflora imbalance in diabetic mice.The specific mechanism needs to be further studied.