hsa_circ_0001785在乳腺癌患者血清中的表达及临床意义研究

A study on the expression and clinical significance of hsa_circ_0001785 in serum of breast cancer patients

目的探讨hsa_circ_0001785在乳腺癌患者血清中的表达及临床意义。方法选择2021年9月至2022年3月广州医科大学附属第五医院甲乳外科收治的乳腺癌患者43例(乳腺癌组),乳腺良性肿瘤患者54例(良性肿瘤组),健康体检者45名(健康对照组)。采用实时荧光定量PCR(qPCR)检测其血清hsa_circ_0001785的相对表达量,并进行三组间比较。分析hsa_circ_0001785表达水平与乳腺癌患者临床病理特征的关联性。采用受试者工作特征(ROC)曲线分析hsa_circ_0001785诊断乳腺癌的效能。结果乳腺癌组血清hsa_circ_0001785表达水平显著高于良性肿瘤组和健康对照组(P<0.05),良性肿瘤组和健康对照组比较差异无统计学意义(P>0.05)。ROC曲线分析结果显示,血清hsa_circ_0001785具有诊断乳腺癌的应用价值(AUC=0.780,P<0.05),且诊断效能优于癌胚抗原(CEA)、癌抗原125(CA125)。乳腺癌患者血清hsa_circ_0001785的表达水平与其年龄、肿瘤类型、TNM分期、淋巴结受累、远处转移、雌激素受体(ER)、孕激素受体(PR)、人类表皮生长因子受体2(HER2)均无显著关联(P>0.05)。结论hsa_circ_0001785在乳腺癌患者血清中高表达,有作为乳腺癌诊断分子标志物的潜力。

Objective To investigate the expression and clinical significance of hsa_circ_0001785 in serum of breast cancer patients.Methods Forty-three patients with breast cancer(the breast cancer group),and 54 patients with benign breast tumor(the benign tumor group)who were admitted to the Department of Thyroid and Breast Surgery of the Fifth Affiliated Hospital of Guangzhou Medical University from September 2021 to March 2022 were selected,and 45 healthy subjects who received physical examination were selected as the healthy control group.The relative expression level of hsa_circ_0001785 in serum was detected by quantitative real-time polymerase chain reaction(qPCR)and was compared among the three groups.The correlation between the expression level of hsa_circ_0001785 and the clinicopathologic features of breast cancer patients were analyzed.The diagnostic efficiency of hsa_circ_0001785 for breast cancer was analyzed by receiver operating characteristic(ROC)curve.Results The expression level of serum hsa_circ_0001785 in the breast cancer group was significantly higher than that in the benign tumor group and the healthy control group(P<0.05),but there was no significant difference between the benign tumor group and the healthy control group(P>0.05).The results of ROC curve analysis showed that serum hsa_circ_0001785 had the application value for diagnosis of breast cancer[area under the curve(AUC)=0.780,P<0.05],and the diagnostic efficiency of serum hsa_circ_0001785 was better than that of carcinoembryonic antigen(CEA)and cancer antigen 125(CA125).The expression level of serumhsa_circ_0001785 in the patients with breast cancer was not significantly associated with their age,tumor type,TNM stage,lymph node involvement,distant metastasis,estrogen receptor(ER),progesterone receptor(PR)and human epidermal growth factor receptor 2(HER2)(P>0.05).Conclusion Hsa_circ_0001785 is highly expressed in serum of breast cancer patients and has potential as a molecular marker for diagnosis of breast cancer.