反蛋白石光子晶体快速检测人心脏型脂肪酸结合蛋白的技术研究

Technical research on rapid detection of human heart-type fatty acid-binding protein by inverse opal photonic crystal

目的 建立一种用于快速检测人心脏型脂肪酸结合蛋白(heart-type fatty acid-binding protein,H-FABP)的光子晶体(photonic crystal,PC)水凝胶传感检测方法。方法 以甲基丙烯酸(methacrylic acid,MAA)为功能单体,二甲基丙烯酸乙二醇酯(ethylene glycol dimethacrylate,EGDMA)为交联剂制备掺杂金纳米颗粒的反蛋白石PC水凝胶。进一步修饰H-FABP抗体,制得反蛋白石PC传感材料,研究该传感材料对H-FABP的响应性能。结果 在最佳实验条件下,该方法的检测范围为100 pg/mL~1μg/mL,检出限为11.2 pg/mL,响应时间为8 min,且具有良好的特异性。模拟实际样品的加标回收实验结果表明,该方法具有良好的准确性。与酶联免疫吸附测定试剂盒方法相比,该方法灵敏度较高,检测范围较广。结论 该传感检测方法结合了PC的光学特性与水凝胶的响应特点,实现了对H-FABP的快速检测,为急性冠脉综合征的生物标志物的早期筛查提供技术支撑。

Objective To develop a photonic crystal(PC) hydrogel sensing detection method for the rapid detection of human hearttype fatty acid-binding protein(H-FABP). Methods Inverse opal PC hydrogels doped with gold nanoparticles were prepared using methacrylic acid(MAA) as the functional monomer and ethylene glycol dimethacrylate(EGDMA) as the cross-linking agent. The H-FABP antibody was further modified to prepare an inverse opal PC sensing material,and the response performance of the sensing material to H-FABP was studied. Results Under the optimal experimental conditions,the detection range of this method was 100 pg/mL-1 μg/mL,the detection limit was 11.2 pg/mL,the response time was 8 min,and the method had good specificity.The experimental results of spiked recovery simulating actual samples showed that the method had good accuracy. Compared with the ELISA kit method,this method had higher sensitivity and wider detection range. Conclusion The sensing detection method combines the optical properties of PC and the response characteristics of hydrogels,realizes the rapid detection of H-FABP,and provides technical support for the early screening of biomarkers of acute coronary syndrome.