陆地棉GhERL1基因的鉴定及生物信息学分析

Identification and Bioinformatics Analysis of GhERL1 Gene in Upland Cotton

棉花是我国重要的经济作物,其生长发育过程中易受非生物胁迫和病菌危害。而ERL1基因是植物体内重要的信号转导因子,其在植物抗逆和生长发育过程中有重要作用。通过分析不同胁迫模式下陆地棉ERL1基因的表达特征,了解其在逆境胁迫中具有的功能,以期为分子育种创新种质提供基因资源。本研究以ERECTA家族成员中的ERL1为研究目标,采用生物信息学方法分析陆地棉GhERL1的理化性质、蛋白质结构、亚细胞定位建模、基因的组织特异性表达和蛋白互作调控网络。生物信息学分析表明陆地棉GhERL1含有998个氨基酸残基,理论等电点为5.76,具有典型的LRR富集区,属于稳定蛋白。蛋白质二级结构以α螺旋和无规卷曲为主,亚细胞定位预测GhERL1分布在细胞膜。GhERL1在子叶、纤维、胚珠、根、茎和叶组织均有表达,在根和茎组织的表达量高于其他组织。在不同时间的冷胁迫、热胁迫、干旱胁迫和盐胁迫下,GhERL1基因的表达存在差异性。而在蛋白互作调控网络中,GhERL1和参与气孔发育、生物信号传递、细胞周期调控相关的蛋白构成共表达网络。陆地棉GhERL1的鉴定及不同胁迫模式下的表达分析为棉花生长发育过程中的非生物胁迫研究提供了重要的线索。

Cotton is an important economic crop in China,which is susceptible to abiotic stress and pathogen damage during its growth and development.The ERL1 gene is an important signal transduction factor in plants,which plays an important role in plant stress resistance and growth and development.By analyzing the expression characteristics of upland cotton ERL1 gene under different stress modes,we can understand its function in adversity stress,in order to provide genetic resources for molecular breeding innovative germplasm.This study targeted ERL1 in ERECTA family members,bioinformatics methods were used to analyze the physicochemical properties,protein structure,subcellular localization modeling,gene tissue-specific expression and protein interaction regulatory network of GhERL1 in upland cotton.Bioinformatics analysis showed that GhERL1 of upland cotton contains 998 amino acid residues,the theoretical isoelectric point is 5.76,has a typical LRR-enriched region,and belongs to a stable protein.The protein secondary structure is dominated byα-helix and random coil,and the subcellular localization predicts that GhERL1 is distributed in the cell membrane.GhERL1 was expressed in cotyledons,fibers,ovules,roots,stems and leaf tissues,and the expression level in root and stem tissues was higher than other tissues.There were differences in the expression of GhERL1 gene under different times of cold stress,heat stress,drought stress and salt stress.In the protein interaction regulatory network,GhERL1 and proteins involved in stomatal development,biological signal transmission,and cell cycle regulation constitute a co-expression network.The identification of GhERL1 in upland cotton and its expression analysis under different stress modes provide important clues for the study of abiotic stress during cotton growth and development.