化瘀通络灸对血管性痴呆大鼠髓鞘再生相关蛋白表达的影响

Effects of Blood Stasis-Removing and Collateral-Dredging Moxibustion on Expression of Myelin Regeneration-Related Proteins in Rats with Vascular Dementia

目的观察化瘀通络灸对血管性痴呆(vascular dementia,VD)大鼠胼胝体髓鞘相关蛋白表达的影响,为艾灸治疗VD提供依据。方法Wistar大鼠经Morris水迷宫筛选后随机分为假手术组、模型组、艾灸组、西药组,每组12只。采用双侧颈总动脉永久结扎法制备VD大鼠模型。假手术组和模型组不作其他任何处理;艾灸组取“百会”“大椎”“神庭”悬灸20 min,每日1次;西药组用氯马斯汀(H1受体拮抗剂)溶液灌胃,每日2次。以上4组干预7 d为1个疗程,共3个疗程。采用Morris水迷宫检测干预前后大鼠学习记忆能力;免疫荧光染色和Western blot法检测大鼠胼胝体区髓鞘相关糖蛋白(myelin associated glycoprotein,MAG)、髓鞘碱性蛋白(myelin basic protein,MBP)表达水平。结果与假手术组比较,模型组大鼠逃避潜伏期延长(P<0.05);与模型组比较,艾灸组、西药组逃避潜伏期均显著缩短(P<0.05)。与假手术组比较,模型组胼胝体区MAG、MBP平均荧光强度显著降低(P<0.05);与模型组比较,艾灸组MAG、MBP平均荧光强度显著增强(P<0.05);与西药组比较,艾灸组MAG平均荧光强度显著增强(P<0.05),MBP平均荧光强度差异无统计学意义(P>0.05)。与假手术组比较,模型组胼胝体区MAG、MBP表达水平下降(P<0.05);与模型组比较,艾灸组MAG、MBP表达水平增加(P<0.05);与西药组比较,艾灸组MAG表达水平增加(P<0.05),MBP表达水平差异无统计学意义(P>0.05)。结论化瘀通络灸可改善VD大鼠的学习记忆能力,促进VD大鼠髓鞘相关蛋白MAG、MBP的合成,促使髓鞘再生。

Objective To investigate the effects of blood stasis-removing and collateral-dredging moxibustion on the expression of myelin-associated glycoprotein(MAG)and myelin basic protein(MBP)in the corpus callosum of rats with vascular dementia(VD),and to provide a basis for treating VD with moxibustion.Methods Wistar rats selected by the Morris water maze test were randomly divided into sham group,model group,moxibustion group,and Western medicine group,with 12 rats in each group.A rat VD model was prepared by permanent ligation of bilateral common carotid arteries.The sham and model groups had no additional treatment.The moxibustion group received suspended moxibustion at the"Baihui","Dazhui",and"Shenting"points for 20 min,once a day.The Western medicine group was given clemastine(H1-receptor antagonist)solution by gavage twice a day.With seven days as a course of treatment,the treatments lasted for three courses.The Morris water maze test was used to detect the learning and memory ability of rats before and after intervention.The expression of MAG and MBP in the corpus callosum of rats was measured by immunofluorescence assay and Western blot.Results Compared with that in the sham group,escape latency was significantly prolonged in the model group(P<0.05).The moxibustion group and Western medicine group had significantly shortened escape latencies than the model group(P<0.05).The mean fluorescence intensities of MAG and MBP in the corpus callosum were significantly decreased in the model group compared with the sham group(P<0.05).Compared with the model group,the moxibustion group showed significantly increased mean fluorescence intensities of MAG and MBP(P<0.05).Compared with the Western medicine group,the moxibustion group showed a significantly increased mean fluorescence intensity of MAG(P<0.05),with no significant difference in the mean fluorescence intensity of MBP(P>0.05).The expression of MAG and MBP in the corpus callosum was significantly decreased in the model group than in the sham group(P<0.05).MAG and MBP expression in the moxibustion group was significantly increased compared with that in the model group(P<0.05).Compared with the Western medicine group,the moxibustion group showed significantly increased MAG expression(P<0.05),with no significant difference in MBP expression(P>0.05).Conclusion Blood stasis-removing and collateral-dredging moxibustion can improve the learning and memory ability of rats with VD,and promote the synthesis of the myelin-related proteins MAG and MBP to facilitate myelin regeneration in rats with VD.