富硒平菇粉的抗癌活性评价及富硒代糖面包的研制

Anticancer Activity Evaluation of Selenium-enriched Pleurotus ostreatus Fungus Powder and Development of Selenium-enriched Sugar Substitute Bread

该研究采用不同总硒浓度的富硒平菇粉联合5-FU处理肝癌细胞HepG2,应用CCK-8法、流式细胞术双染法探讨富硒平菇粉联合5-FU的抑癌效果,并以富硒平菇粉为原料研制一款富硒代糖面包,为富硒功能食品研发提供参考依据。结果表明,富硒平菇粉对肝癌细胞HepG2增殖有抑制作用,抑制率范围为13.89%~27.39%;不同总硒浓度的富硒平菇粉联合5-FU干预HepG2肝癌细胞36 h后,凋亡细胞数量呈硒作用浓度依赖性增高(p<0.05);总凋亡率分别是26.36%~49.72%,且凋亡率高于5-FU组(p<0.05)。富硒代糖面包的最佳加工工艺配方为:200 g面粉中富硒平菇粉添加量为0.06 g,复配代糖添加量为25 g,烘烤温度为上下火175℃,烘烤时间为20 min,总硒含量为(0.17±0.01)mg/kg。综上所述,采用不同总硒浓度的富硒平菇粉联合5-FU处理肝癌细胞HepG2,发现富硒平菇粉联合5-FU可抑制HepG2肝癌细胞生长,起到增效解毒的作用,并成功研制出富硒代糖面包的最佳工艺,具有较好的研究和应用价值。

Selenium(Se)-enriched Pleurotus ostreatus fungus powder with different total Se concentrations was used in combination with 5-FU to treat HepG2 hepatocellular carcinoma cells.The CCK-8 and flow cytometry double staining methods were applied to investigate the tumor suppressive effect of Se-enriched P.ostreatus fungus powder combined with 5-FU.The Se-enriched P.ostreatus fungus powder was then used as the raw material to develop a Se-enriched sugar substitute bread,providing scientific evidence for the development of Se-enriched functional foods.The results demonstrate that the Se-enriched P.ostreatus fungus powder had an inhibitory effect on the proliferation of HepG2 hepatocellular carcinoma cells,with the inhibition rate ranging from 13.89%to 27.39%.The number of apoptotic cells in HepG2 hepatocellular carcinoma increased in a concentration-dependent manner after treatment with different total Se concentrations of Se-rich P.ostreatus fungus powder combined with 5-FU for 36 h(p<0.05).The total apoptosis rates were 26.36%~49.72%,respectively,and the apoptosis rates were higher than those in the 5-FU group(p<0.05).The optimal processing formula of Se-enriched sugar-substitute bread was as follows:0.06 g Se-enriched P.ostreatus fungus powder was added to 200 g flour with 25 g compound sugar substitute and baked at 175°C for 20 min;the total Se content was 0.174±0.011 mg/kg.In conclusion,when HepG2 hepatoma cells were treated with Se-enriched P.ostreatus fungus powder at different total Se concentrations combined with 5-FU,HepG2 hepatoma cell growth was inhibited,increasing the efficiency of detoxification.The optimal processing formula of Se-enriched sugar substitute bread,which has various research and application values,was also developed.