肝细胞癌相关固醇-O酰基转移酶1基因的生物信息学分析

Bioinformatics analysis of sterol O-acyltransferase 1 gene related to hepatocellular carcinoma

目的使用生物信息学工具对肝细胞癌相关固醇-O酰基转移酶1(sterol O-acyltransferase 1,SOAT1)的结构、功能进行预测分析,以了解其作为S-Ⅲ亚型标记物以及治疗靶标的相关作用机制。方法使用NCBI、STRING、Protscale、SignalP、TMHMM、PSORT、SOPMA、SWISS-MODEL、NetNGlyc、NetOGlyc、Netphos、ProtParam等数据库或软件,对SOAT1的结构、功能、蛋白相互作用等进行预测分析。结果SOAT1编码的蛋白质为疏水性蛋白,稳定性较好,属于非经典途径蛋白,具有8个跨膜区域,主要分布于细胞膜。SOAT1在多种组织均有表达,但绝大多数位于肾上腺。具有多个磷酸化位点,主要参与胆固醇的合成以及分解代谢过程。结论通过生物信息学分析SOAT1的结构以及功能,发现SOAT1脂质的合成以及分解代谢途径发挥重要作用,脂质的表达与癌症的发展密切相关。因此可通过调节SOAT1基因的表达从而实现肝细胞癌的治疗。

Objective To predict the structure and function of sterol O-acyltransferase 1(SOAT1)related to hepatocellular carcinoma(HCC)by using bioinformatics tools,in order to understand its mechanism as the marker and therapeutic target of S-Ⅲsubtype.Methods The structure,function and protein interaction of SOAT1 were predicted and analyzed by using databases or softwares such as NCBI,STRING,Protscale,SignalP,TMHMM,PSORT,SOPMA,SWISS-MODEL,NetNGlyc,NetOGlyc,Netphos and ProtParam.Results The protein encoded by SOAT1 was a hydrophobic protein with good stability,which was a nonclassical pathway protein with 8 transmembrane regions,mainly distributed among the cell membrane.SOAT1 was expressed in many tissues,while most of them in the adrenal gland,which showed multiple phosphorylation sites and was mainly involved in the synthesis and catabolism of cholesterol.Conclusion Bioinformatics analysis of structure and function of SOAT1 showed that SOAT1 lipid synthesis and catabolism pathways played an important role,and lipid expression was closely related to the development of cancer,indicating that the treatment of HCC may be achieved by regulating the expression of SOAT1 gene.