黄芪多糖对食管癌细胞增殖与凋亡的影响及其分子机制研究

Effects of astragalus polysaccharide on the proliferation and apoptosis of esophageal carcinoma cells and its molecular mechanism

目的研究黄芪多糖(APS)对食管癌细胞的调控作用。方法分别在活体水平和细胞水平添加不同浓度的APS,研究其对裸鼠成瘤及食管癌细胞增殖和凋亡的影响。活体水平:向裸鼠体内注射食管癌细胞和不同浓度APS后,检测肿瘤的生长趋势和抑瘤率;细胞水平:采用EdU染色和流式细胞术检测不同浓度APS处理后,食管癌细胞的细胞周期和凋亡情况。qPCR、Western blotting和Co-IP试验探讨APS对食管癌进行调控的分子机制。结果向裸鼠食管癌移植瘤模型体内注射APS可抑制肿瘤的增殖,且随着剂量的增加,其抑癌效果亦增强。APS可抑制食管癌细胞增殖,促进其凋亡。TP73、FBXW7的表达随APS浓度的增加而增加,Ki67、BCL-2的表达随APS浓度的增加而降低。Co-IP试验发现,APS能够与TP73蛋白结合。过表达TP73可抑制食管癌细胞增殖并促进其凋亡,沉默TP73则与之相反。结论本研究揭示了APS对食管癌的调控作用及其可能的分子机制,为食管癌的治疗及APS的应用提供了一定的研究基础。

Objective To explore the regulation of astragalus polysaccharide(APS)on esophageal cancer cells.Meth⁃ods Different concentrations of APS were taken to figure out their effects on tumor formation and on the cell proliferation and apoptosis in nude mice in vivo and in vitro.In vivo,we first made an injection of esophageal cancer cells to the nude mice,then the tumor growth trends and its suppression rates were detected after the other injection of APS in different con-centrations.In vitro,the cell cycle and apoptosis rates of esophageal cancer cells were detected through EdU staining and flow cytometry after the treatment of different concentrations of APS.The qPCR,Western blotting and Co-IP assay were used to investigate the molecular mechanism of APS regulation on esophageal cancer.Results The tumor proliferation was inhibited after APS treatment and the effect of tumor suppression was enhanced with the dose raising.APS can reduce the proliferation of esophageal cancer cell and promote its apoptosis.The expression of TP73 and FBXW7 was increased along with the raising concentrations of APS,while the expression of Ki67 and BCL-2 was decreased.The Co-IP results revealed that APS was able to bind to the protein of TP73.Overexpressed TP73 could suppress the cell proliferation and promote the apoptosis of esophageal cancer cells,while silenced TP73 did the opposite.Conclusion This study revealed the regulatory effects of APS on esophageal cancer and its possible molecular mechanism,which provided a certain research basis for the treatment of esophageal cancer and the application of APS.