miRNA-154在皮肤鳞状细胞癌中的表达及其临床意义

Expression and clinical significance of miRNA-154 in cutaneous squamous cell carcinoma

目的分析miRNA-154在皮肤鳞状细胞癌(CSCC)中的表达水平以及其在CSCC发展过程中的临床意义。方法采用RT-PCR法和原位杂交法检测98例CSCC组织和60例正常皮肤组织中miRNA-154的表达水平。研究对入组患者均进行了5年的跟踪随访,对患者的生存情况进行统计和总结,同时对miRNA-154表达水平和CSCC患者预后的关系进行进一步分析。结果CSCC组织中miRNA-154相对表达量为(0.57±0.09),明显低于其在正常组织中的表达量(1.56±0.02),差异有统计学意义(P<0.001)。CSCC组织中miRNA-154表达的平均光密度为(63.26±10.08),明显低于正常组织的(109.56±30.05),差异有统计学意义(P<0.001)。CSCC组织中miR‐NA-154表达水平越低,则临床分期越高、肿瘤分化程度越低,同时越容易发生淋巴结转移,差异均有统计学意义(P<0.05)。miRNA-154高表达组患者的总生存期显著长于miRNA-154低表达组(41.9个月vs.31.4个月,P<0.001)。Cox回归分析结果显示,miRNA-154低表达、肿瘤低分化、高临床分期及淋巴结转移与CSCC患者死亡预后显著相关(P<0.05)。结论在CSCC发生发展过程中,miRNA-154是重要的抑癌基因,其表达水平与肿瘤分化程度呈负相关,与临床分期、淋巴结转移以及生存期呈正相关,可能是潜在的抗肿瘤生物治疗靶点。

Objective To analyze the expression level of miRNA-154 in cutaneous squamous cell carcinoma(CSCC)and its clinical significance in the development of CSCC.Methods RT-PCR and in situ hybridization were used to detect the expression of miRNA-154 in 98 cases of CSCC and 60 cases of normal skin.All the patients were followed up for 5 years,and the survival rate of the patients were counted and summarized.The relationship between the expression level of miRNA-154 and the prognosis of CSCC patients was further analyzed.Results The relative expression of miRNA-154 in CSCC tissues(0.57±0.09)was significantly lower than in normal tissues(1.56±0.02)(P<0.001).The average optical density of miRNA-154 expression in CSCC tissues(63.26±10.08)was significantly lower than in normal tissues(109.56±30.05)(P<0.001).The lower the level of miRNA-154 expression was in CSCC tissues,the higher the clinical stage,the lower the tumor differentiation,and the more prone to lymphatic metastasis(P<0.05).The overall survival rate of patients with high expression of miRNA-154 was significantly higher than that of patients with low expression of miRNA-154(41.9 months vs.31.4 months,P<0.001).Cox regression analysis showed that the low expression of miRNA-154,low tumor differentiation,high clinical stage,and lymphatic metastasis were related to the prognosis of death in patients with CSCC(P<0.05).Conclusion miRNA-154 is an important tumor suppressor gene in the occurrence and development of CSCC.The expression of miRNA-154 showed a negative relationship with tumor differentiation,and a positive relationship with the clinical stage,lymphatic metastasis and survival rate of CSCC patients.miRNA-154 may be a potential target for anti-tumor biotherapy of CSCC.