枯草芽孢杆菌262XY2'菌肥对番茄幼苗生长及生理特性的影响

Bacillus subtilis 262XY2'Bacterial Fertilizer:Effects on the Growth and Physiological Characteristics of Tomato Seedlings

旨在确定枯草芽孢杆菌262XY2´菌肥的最佳施用浓度并为其实际应用提供理论依据。以番茄和枯草芽孢杆菌262XY2´菌肥为试材,以形态观察和分光光度计测定等方法测定番茄生长与生理指标。结果显示0.5%~2%菌肥处理的生长生理指标最佳。出苗率、株高、茎粗、叶面积、地上地下部鲜干质量、壮苗指数、生长函数、根冠比、叶绿素a、b、类胡萝卜素含量、根系活力和可溶性蛋白含量最高值分别比CK高11.115%、14.589%、35.616%、91.704%、49.369%、48.330%、86.634%、85.714%、71.429%、50.000%、59.164%、86.887%、156.827%、10.589%、7.035%和54.294%,-20℃和40℃处理后相对电导率最低值分别比CK低6.483%和3.116%。施用0.5%~2%枯草芽孢杆菌262XY2´菌肥可促进番茄生长,其中0.5%菌肥处理组对番茄促生效果最为显著。

The objectives of this study are to determine the optimal application concentration of Bacillus subtilis 262XY2'bacterial fertilizer and provide a theoretical basis for its practical application.Tomato and Bacillus subtilis 262XY2'bacterial fertilizer were used as test materials,and the growth and physiological indexes of tomato were determined by morphological observation and spectrophotometer determination.The results showed that 0.5%-2%bacterial fertilizer treatment had the best growth physiological indexes.The ratio of seedling emergence rate,plant height,stem diameter,leaf area,fresh and dry weight of aboveground and underground parts,strong seedling index,growth function,root-shoot ratio,the content of chlorophyll a and b,carotenoid content,root vigor and soluble protein content were 11.115%,14.589%,35.616%,91.704%,49.369%,48.330%,86.634%,85.714%,71.429%,50.000%,59.164%,86.887%,156.827%,10.589%,7.035%and 54.294%higher than those of CK,respectively,and the lowest value of relative conductivity after treatment at-20℃and 40℃were 6.483%and 3.116%lower than that of CK,respectively.In summary,the application of 0.5%-2%Bacillus subtilis 262XY2'bacterial fertilizer can promote tomato growth,and the 0.5%bacterial fertilizer treatment group has the most significant effect on tomato growth.