胰岛素样生长因子-1对缺氧心肌细胞凋亡的作用

Effect of insulin-like growth factor-1 on apoptosis of hypoxic cardiomyocytes

目的:研究胰岛素样生长因子-1(IGF-1)对缺氧心肌细胞凋亡的作用。方法:培养原代心肌细胞,并通过α-actin鉴定细胞纯度。实验分为正常组、缺氧组、IGF-1处理组共三组,缺氧组细胞在更换无血清培养基培养24h后被置于缺氧培养箱缺氧培养24h,IGF-1处理组在缺氧模型前1h给与IGF-1。通过TUNEL染色检测各组细胞凋亡率,蛋白免疫印迹检测凋亡蛋白caspase3的表达变化。结果:缺氧后心肌细胞出现显著的凋亡,TUNEL凋亡率显著升高(P<0.05),caspase3蛋白表达显著升高(P<0.05),IGF-1处理可以降低TUNEL凋亡率(P<0.05)和caspase3蛋白表达(P<0.05)。结论:IGF-1可以抑制缺氧心肌细胞的凋亡。

Objective:To study the effect of insulin-like growth factor-1(IGF-1)on apoptosis of hypoxic cardiomyocytes.Methods:primary myocardial cells were cultured andα-Actin was used to identify cell purity.The experiment was divided into three groups:normal group,hypoxia group and IGF-1 treatment group.The cells in the hypoxia group were placed in the hypoxia incubator for 24hours after changing the serum-free medium.The cells in the IGF-1 treatment group were given IGF-11 h before the hypoxia model.The apoptosis rate was detected by TUNEL staining,and the expression of apoptotic protein caspase3 was detected by Western blot.Results:after hypoxia,cardiomyocytes showed significant apoptosis,TUNEL apoptosis rate increased significantly(P<0.05),and caspase-3 protein expression increased significantly(P<0.05).IGF-1 treatment could reduce TUNEL apoptosis rate(P<0.05)and caspase-3 protein expression(P<0.05).Conclusion:IGF-1 can inhibit the apoptosis of hypoxic cardiomyocytes.