细菌RND外排泵的结构与作用机制研究进展

Research progress in the structure and function of RND efflux pump

细菌RND(resistance-nodulation-cell division)外排泵是一类由内膜转运蛋白、周质融合蛋白、外膜外排蛋白组成的蛋白复合体,能够将抗菌药物和多种小分子化合物如毒素、染料、洗涤剂、脂质、群感信号分子等排出细菌细胞外。通过影响RND外排泵的组装和功能,可逆转多重耐药性的发生与防止耐药性的发展。细菌具有多种外排泵,本文以RND外排泵为代表,介绍与其结构以及外排药物的转运和调控机制的最新进展。

RND(resistance-nodulation-cell division)efflux pump consists of inner membrane protein,periplasmic membrane fusion protein,and outer membrane protein.It pumps out a wide spectrum of antibiotics and leads to development of multi-drug resistance of host cells.Substrates of RND efflux pump are not limited to antibiotics,but also small molecules such as toxins,dyes,lipids,and quorum sensing signals.Due to the importance of RND efflux pump in multi-drug resistance,one can affect and inhibit the assembly and function of RND efflux pump to prevent and/or reserve multidrug resistance.In this article,the important and latest research progress of the structure as well as the transportation and regulation mechanism of representative RND efflux pumps is reviewed.