聚醚胺ED900-单宁酸构建表面微结构化复合涂层及其细胞相容性的研究

Construction of microstructured composite coatings based on polyetheramine ED900-tannic acid and cytocompatibility study

为改善医用材料的生物相容性并赋予材料表面一定的生物学功能,本文以单宁酸(TA)和聚醚胺ED900为组装单元,通过层层自组装的方法制备了复合涂层。采用纳米粒度仪、Zeta电位分析仪、紫外分光光度计、红外光谱、石英晶体微天平(QCM-D)、扫描电子显微镜等仪器对ED900-TA复合溶液行为及复合涂层的理化性质进行表征。通过细胞实验考察了涂层对细胞行为的影响。利用1,1-二苯基-2-三硝基苯肼(DPPH)法和总抗氧化能力检测试剂盒(FRAP)法评价了涂层的抗氧化性。最后,分别通过琼脂糖插入实验和细胞培养液浸泡研究涂层的稳定性。结果表明:ED900-TA涂层具有良好的细胞相容性和抗氧化性,表面的微结构呈现调控亲/憎细胞的能力。此外,涂层在模拟植入的过程未出现脱落。并在细胞培养条件下,涂层形貌在21天的评价周期内无显著变化。该复合涂层为生物材料表面多功能化提供了新思路。

To improve biocompatibility of biomaterials and endow the surface with biological functions,a new type of composite coatings was developed using the natural biomacromolecule tannic acid(TA)and polyetheramine ED900 through a layer-by-layer self-assembly method.The behavior of ED900-TA complexation in water and the physical/chemical properties of the ED900-TA coatings were characterized using nanoparticle sizer,Zeta potential analyzer,UV-vis spectrophotometer,FTIR spectrometer,quartz crystal microbalance(QCM-D),and SEM.The effect of coatings on cell behavior was investigated in vitro.The anti-oxidative property was measured by 1,1-diphenyl-2-trinitrophenylhydrazine(DPPH)and total antioxidant capacity assay kit(FRAP)assays.Finally,coating stability was evaluated by agarose insertion and incubation in cell culture medium.The results show the coatings being biocompatible and anti-oxidative.Depending on the surface microstructures,the coatings can be cytophilic or cytophobic.Besides,the coatings can withstand the shear force of insertion and the morphology is maintained up to 21 days in culture medium.This composite coating provides a new option for surface functionalization of biomaterials.