超声波-微波协同提取苹果渣多酚工艺优化及其性能评价

Optimization of Ultrasonic-Microwave Synergistic Extraction of Polyphenols from Apple Residue and Its Property Evaluation

为获得更高的苹果多酚提取效率,文章通过单因素和正交实验优化了超声波-微波协同提取苹果渣中苹果多酚的最佳工艺条件,并对纯化后的苹果多酚的抗氧化和体外模拟消化性能进行了检测。结果表明,超声波-微波协同提取苹果渣中苹果多酚的最优工艺参数为:微波功率200 W、超声波功率475 W、乙醇体积分数60%、料液比1:25,此条件下得到苹果多酚得率为(96.98±0.23)mg/g;在苹果多酚粗提液浓度为1.5 mg/mL、吸附时上样流速为2.0 BV/h、解吸时洗脱液的流速为3.0 BV/h条件下,苹果多酚在大孔树脂中的吸附量为2.73 mg/g,吸附率为91.33%,解吸率为78.46%,最终得到的苹果多酚纯度为73.24%;此苹果多酚的DPPH·自由基清除率最高为70.62%,SC_(50)值为15.85 mg/L;在体外模拟胃液中的释放速率2 h内为73.47%,在模拟肠液中释放速率5 h内为94.28%。实验结果表明,超声波-微波协同提取技术可以高效提取苹果渣中的苹果多酚。

In order to obtain a higher extraction efficiency of apple polyphenols,the optimal extraction conditions of apple polyphenols from apple residue using ultrasonic and microwave co-extraction technology were optimized by single factor and orthogonal experiments.The antioxidant activity and simulated digestion in vitro properties of apple polyphenols purified by NKA-9 macroporous adsorption resin were also studied in this work.The results showed that the optimal extraction parameters were as follows:microwave power was 200 W,ultrasonic power was 475 W,ethanol concentration was 60%,solid-liquid ratio was 1:25.Under these conditions,the polyphenol yield reached(96.98±0.23)mg/g.The adsorption capacity of apple polyphenols in macroporous resin was 2.73 mg/g,the adsorption and desorption rate was 91.33%and 78.46%,respectively,and the resulted purity of apple polyphenols was 73.24%under the conditions of 1.5 mg/mL crude extract concentration,2.0 BV/h loading flow rate and 3.0 BV/h desorption flow rate.The highest DPPH·scavenging activity of apple polyphenols in this work was 70.62%,SC_(50)value was 15.85 mg/L,and the release rate of apple polyphenols was 73.47%within 2h in simulated gastric juice and 94.28%within 5 h in simulated intestinal juice in vitro.The results showed that ultrasonic-microwave co-extraction technology could effectively extract apple polyphenols from apple residue.