‘鄞红’葡萄HDR基因的克隆与生物信息学分析

Cloning and Bioinformatics Analysis of HDR Gene from’Yinhong’Grape

4-羟基-3-甲基丁-2-烯基二磷酸还原酶(HDR)作为甲基赤藓醇磷酸(MEP)途径的关键酶,在植物萜类物质合成过程中起到极为重要的作用。为了了解HDR基因在植物体内发挥的特殊功能,以‘鄞红’葡萄为材料,利用RT-PCR等技术克隆了HDR基因全长,并对该基因进行生物信息学分析。结果显示,HDR基因全长为1143 bp,编码380个氨基酸。通过多种软件比对分析,‘鄞红’葡萄与柑橘(Citrus sinensis)、南瓜(Manihot esculenta)、猕猴桃(Actinidia deliciosa)、黄瓜(Cucumis sativus)的相似性高达80%以上,亲缘关系较近。该结果为进一步研究‘鄞红’葡萄HDR的功能以及揭示该基因在植物萜类物质合成过程中发挥的作用提供了一定基础。

4-hydroxy-3-methylbut-2-enyl-diphosphate reductase(HDR)is the the key enzyme of MEP pathway which plays an important role in the synthesis of plant terpenoids.In order to understand the special functions of HDR gene in plants,the full length of HDR gene was cloned from’Yinhong’grape by RT-PCR and bioinformatics analysis.The results showed that the sequence of HDR gene was 1143 bp which encoded 380 amino acids.Through a variety of software analysis and alignment showed that the similarity of’Yinhong’grape with Citrus sinensis,Manihot esculenta,Actinidia deliciosa and Cucumis sativus was more than 80%.The results lay a foundation for further study on the function of HDR in’Yinhong’grape and reveal the role of HDR gene in plant terpenoid synthesis.