KT和TDZ对药用植物山乌龟组织培养的影响

Effects of KT and TDZ on the Tissue Culture of the Medicinal Plant Stephania epigaea

为了快速获得药用植物山乌龟完整植株,优化组织快繁技术,本研究以山乌龟嫩茎段为实验材料,研究激动素(kinetin,KT)和噻苯隆(N’-phenyl-N’-1,2,3-thidiazol-5-yl-urea,TDZ)对山乌龟不定芽诱导、增殖和生根培养的影响,以获得最佳的培养条件。结果表明,0.2%次氯酸钠(NaClO)浸泡15 min为最适合山乌龟嫩茎段消毒的方法,污染率仅为8.31%;最有利于山乌龟不定芽萌发的培养基为MS+6-BA1.0 mg/L+NAA0.2 mg/L+KT 0.5 mg/L+聚乙烯吡咯烷酮(polyvinyl pyrrolidone,PVP)1 g/L+琼脂7 g/L+蔗糖30 g/L,诱导不定芽数为8.42;不定芽增殖最佳培养配方为MS+KT 0.8 mg/L+NAA 0.5 mg/L+活性炭1 g/L+琼脂7 g/L+蔗糖30 g/L,增殖系数10.87;最适生根配方为1/2MS+KT 0.2 mg/L+IBA 1.0 mg/L+NAA 0.2 mg/L+琼脂7 g/L+蔗糖15 g/L。本研究优化了山乌龟组培快繁技术,提高了繁殖系数,对其规模化生产及缓解供求矛盾具有重要意义。

In order to culture the complete plant of the medicinal plant Stephania epigaea more efficiently,the tissue culture and rapid propagation technologies were optimized.Through applying the plant growth hormone kinetin(KT)and N’-phenyl-N’-1,2,3-thidiazol-5-yl-urea(TDZ)on the adventitious bud of Stephania epigaea,we have investigated the effects on bud induction,multiplication and rooting culture,aiming to exploring the optimal culturing conditions.Our results showed that the best disinfection condition was to soak the plant in 0.2%NaClO solution for 15 min,from which the pollution rate was minimized to 8.31%;Under the culturing condition of MS+6-BA 1.0 mg/L+NAA 0.2 mg/L+KT 0.5 mg/L+(Polyvinyl Pyrrolidone)PVP 1 g/L+Agar 7 g/L+sucrose 30 g/L,the number of adventitious buds induced has reached to 8.42,suggesting the best medium composition.Under the culturing condition of MS+KT 0.8 mg/L+NAA 0.5 mg/L+activated Carbon 1 g/L+Agar 7 g/L+sucrose 30 g/L,the highest multiplication coefficient(10.87)of the adventitious bud was witnessed,indicating the best medium condition.Under the culturing condition of 1/2MS+KT 0.2 mg/L+IBA 1.0 mg/L+NAA 0.2 mg/L+Agar 7 g/L+sucrose 15 g/L,the maximum rooting rate was reflected.This study has optimized the system for rapid Stephania epigaea propagation and improved the multiplication coefficient of the adventitious bud,which has demonstrated promising significance to the agricultural production of Stephania epigaea in large-scale.