放射性同位素示踪法研究氟啶虫酰胺在蛋鸡中的排泄

Study on the Excretion of Flonicamid in Laying Hens by Radioisotope Tracer

为了研究氟啶虫酰胺在蛋鸡中的排泄,本研究采用放射性同位素14C标记氟啶虫酰胺,以蛋鸡为试验材料,通过胶囊经口方式给药,并对给药胶囊稳定性进行考察,采用氧化燃烧的方法对粪便中的总放射性残留进行分析,采用液体闪烁计数分析仪(LSC)对鸡蛋及清洗液进行总放射性残留的分析。试验结果显示,在给药过程中胶囊中的氟啶虫酰胺稳定,2次平行样品的放射化学纯度偏差为0.101%。鸡蛋样品的残留量为0.239~1.570 mg/kg,氟啶虫酰胺的总回收量为2.364 mg,占总给药量的3.01%;粪便样品的残留量为4.73~12.70 mg/kg,氟啶虫酰胺的总回收量为69.58 mg,占总给药量的88.8%;粪便清洗液样品的残留量为0.756 mg/kg,氟啶虫酰胺的总回收量为0.675 mg,占总给药量的0.861%;受试物质氟啶虫酰胺的总物质平衡回收率为92.7%。研究表明,氟啶虫酰胺在蛋鸡体内主要通过粪便排泄,鸡蛋中的排泄量较低,并且在蛋鸡其他组织中分布较少。

In this study,the radioisotope 14C-labeled flonicamid was administered in capsules to study the excretion of flonicamid in laying hens.The stability of the administered capsules was investigated.The total radioactive residues in feces were analyzed by oxidative combustion method,and the total radioactive residues in eggs and washing liquid were analyzed by liquid scintillation counting analyzer(LSC).The deviation of the radiochemical purity was 0.101%,which indicated that the test substance in the capsule was stable during the administration.The residues of egg samples were 0.239-1.570 mg/kg,and the total recovery of flonicamid was 2.364 mg,accounting for 3.01%of the total dose administered.The residues of feces samples were 4.73-12.70 mg/kg,and the total recovery of flonicamid was 69.58 mg,accounting for 88.8%of the total dose administered.The residue of the fecal washing liquid sample was 0.756 mg/kg,and the total recovery of flonicamid was 0.675 mg,accounting for 0.861%of the total dose administered.The mass balance recovery rate of the test substance flonicamid was 92.7%.The results showed that flonicamid mainly existed in feces of laying hens,and the excretion in eggs and other tissues of laying hens were low.