基于UPLC-Q-Exactive Focus-MS/MS技术分析藤络宁胶囊中化学成分

Identification of Chemical Constituents in Tengluoning Capsules by UPLC-Q-Exactive Focus-MS/MS

目的:使用超高效液相色谱-四极杆-静电场轨道离子阱联用质谱(UPLC-Q-Exactive Focus-MS/MS)技术对藤络宁胶囊中化学成分进行快速识别和鉴定。方法:色谱柱:Thermo Accucore aQ C18(2.1 mm×150 mm,2.6μm);流动相:甲醇(A)-0.1%甲酸水溶液(B),梯度洗脱(0~45 min,5%~95%A;45.01~50 min,5%A);流速:0.3 ml·min^(-1);柱温:30℃;进样量:3μl。质谱分析采用正、负离子监测模式和高性能电喷雾离子源(HESI),扫描范围m/z 80~1200。结果:从藤络宁胶囊中共鉴定出53个化学成分,包括有机酸类11个、香豆素类21个、生物碱类13个、醌类5个、其他3个。结论:本方法能够对藤络宁胶囊中的化学成分进行快速、全面地分析,可为藤络宁胶囊的药效物质及质量控制研究提供依据。

Objective:To rapidly recognize and identify the chemical components in Tengluoning capsules by ultra-high performance liquid chromatography coupled with Q-Exactive Focus mass spectrometry(UPLC-Q-Exactive Focus-MS/MS).Methods:A Thermo Accucore aQ C18column(2.1 mm×150 mm,2.6μm)was used for the separation.The mobile phase consisted of methanol(A)-0.1%formic acid solution(B)with gradient elution(0-45 min,5%-95%A;45.01-50 min,5%A).The flow rate was 0.3 ml·min^(-1),the column temperature was 30℃,and the injection volume was 3μl.Positive and negative monitoring modes and heated electrospray ion source(HESI)were used for mass spectrographic analysis.The scanning range was m/z 80-1200.Results:Totally 53 chemical constituents of Tengluoning capsules were identified,including 11 organic acids,21 coumarins,13 alkaloids,5 quinones and 3 others.Conclusion:This method can be used to analyze chemical compositions of Tengluoning capsules quickly and comprehensively.This paper provides reference for further studies on pharmacodynamic materials and quality assessment of Tengluoning capsules.