摘要
目的:使用超高效液相色谱-四极杆-静电场轨道离子阱联用质谱(UPLC-Q-Exactive Focus-MS/MS)技术对藤络宁胶囊中化学成分进行快速识别和鉴定。方法:色谱柱:Thermo Accucore aQ C18(2.1 mm×150 mm,2.6μm);流动相:甲醇(A)-0.1%甲酸水溶液(B),梯度洗脱(0~45 min,5%~95%A;45.01~50 min,5%A);流速:0.3 ml·min^(-1);柱温:30℃;进样量:3μl。质谱分析采用正、负离子监测模式和高性能电喷雾离子源(HESI),扫描范围m/z 80~1200。结果:从藤络宁胶囊中共鉴定出53个化学成分,包括有机酸类11个、香豆素类21个、生物碱类13个、醌类5个、其他3个。结论:本方法能够对藤络宁胶囊中的化学成分进行快速、全面地分析,可为藤络宁胶囊的药效物质及质量控制研究提供依据。
Objective:To rapidly recognize and identify the chemical components in Tengluoning capsules by ultra-high performance liquid chromatography coupled with Q-Exactive Focus mass spectrometry(UPLC-Q-Exactive Focus-MS/MS).Methods:A Thermo Accucore aQ C18column(2.1 mm×150 mm,2.6μm)was used for the separation.The mobile phase consisted of methanol(A)-0.1%formic acid solution(B)with gradient elution(0-45 min,5%-95%A;45.01-50 min,5%A).The flow rate was 0.3 ml·min^(-1),the column temperature was 30℃,and the injection volume was 3μl.Positive and negative monitoring modes and heated electrospray ion source(HESI)were used for mass spectrographic analysis.The scanning range was m/z 80-1200.Results:Totally 53 chemical constituents of Tengluoning capsules were identified,including 11 organic acids,21 coumarins,13 alkaloids,5 quinones and 3 others.Conclusion:This method can be used to analyze chemical compositions of Tengluoning capsules quickly and comprehensively.This paper provides reference for further studies on pharmacodynamic materials and quality assessment of Tengluoning capsules.
作者
曲彤
李宁
任慧
崔小敏
胡静
鲁文静
王小彤
陈志永
Qu Tong;Li Ning;Ren Hui;Cui Xiaomin;Hu Jing;Lu Wenjing;Wang Xiaotong;Chen Zhiyong(Shaanxi Academy of Traditional Chinese Medicine,Xi'an 710003,China;School of Pharmacy,Shanghai University of Traditional Chinese Medicine)
出处
《中国药师》
CAS
2022年第12期2158-2165,共8页
China Pharmacist
基金
国家自然科学基金面上项目(编号:81973419)
国家自然科学基金青年项目(编号:81603264)
陕西省中医药管理局青年培优项目(编号:2021-PY-003)
陕西省重点研发计划一般项目(编号:SF-315)
陕西省中医药研究院院级“苗圃培育计划”项目(编号:2021-26)。
关键词
藤络宁胶囊
轨道阱高分辨质谱
化学成分
有机酸类
生物碱类
香豆素类
Tengluoning capsules
Q-Exactive Focus mass spectrometry
Chemical component
Organic acid
Alkaloid
Coumarins