页岩气田含氧管道内硫酸盐还原菌生长特性

Growth Characteristics of Sulfate Reducing Bacteria in Oxygenated Pipeline of Shale Gas Field

为了明确页岩气管线中硫酸盐还原菌的生长特性,采集某页岩气集气管线水样,并富集纯化其中的硫酸盐还原菌(SRB)。提取SRB的DNA并通过基因扩增技术(Polymerase Chain Reaction, PCR)对SRB群落特征进行了描述,定量测试了SRB在溶解氧环境下的生长特性。结果表明:页岩气集气管道内的SRB主要分为Bacteroidota、Firmicutes和Desulfobacterota。溶解氧的浓度对SRB的生长代谢有影响,在0~2.5 mg/L溶解氧下,SRB仍能大量生长繁殖;在4.5 mg/L及以上溶解氧下,SRB的生长代谢被完全抑制,说明在页岩气集气管道内,SRB能在低浓度O2条件下生长繁殖。

For clarifying the growth characteristics of sulfate reducing bacteria in shale gas pipelines, water samples were collected from a shale gas-gathering pipeline, and the sulfate reducing bacteria(SRB) in the samples were enriched and purified. The DNA of SRB was extracted, and then the gene amplification technology(Polymerase Chain Reaction, PCR) was utilized to describe the characteristics of SRB community. Furthermore, the growth characteristics of SRB in dissolved oxygen environment were quantitatively tested. Results showed that the SRB in shale gas gathering pipeline were mainly Bacteroidota, Firmicutes and Desulfobacterota. The concentration of dissolved oxygen had an impact on the growth and metabolism of SRB. Under a dissolved oxygen concentration of 0~2.5 mg/L, SRB could still grow and reproduce in large quantities. Furthermore, under a dissolved oxygen concentration of 4.5 mg/L and above, the growth and metabolism of SRB were completely inhibited, indicating that SRB were able to grow and reproduce under low O2concentration in the shale gas gathering pipeline.