思茅松毛虫成虫肠道细菌多样性

Study on the Diversity of Gut Bacteria from Adults of Dendrolimus kikuchii

【目的】研究野外采集的思茅松毛虫(Dendrolimu kikuchii)成虫的肠道细菌多样性,为思茅松毛虫的防治和云南松(Pinus yunnanensis)等树木的保护奠定基础。【方法】分别提取思茅松毛虫雄蛾和雌蛾的肠道总DNA,基于Illumina NovaSeq(PE250)平台运用高通量技术对肠道细菌16S r DNA基因V3~V4区进行测序和分析,分析雄虫和雌虫的肠道菌群的结构多样性。【结果】从思茅松毛虫成虫中共获得1 278个操作分类单元(OTUs),涵盖28个门75个纲173个目296个科550个属。在门水平上,雄蛾和雌蛾的优势菌为变形菌门(Proteobacteria,雌81.27%/雄81.17%);在科水平上,雄蛾的优势菌科是鞘脂单胞菌科(Sphingomonadaceae,49.16%)和丛毛单胞菌科(Comamonadaceae,19.09%),雌蛾的优势菌科是欧文氏菌科(Erwiniaceae,34.09%)和丛毛单胞菌科(Comamonadaceae,16.68%);在属水平上,雄蛾的优势菌属是鞘氨醇单胞菌属(Sphingomonas,48.09%),雌蛾的优势菌属是泛生菌属(Pantoea,31.58%)。思茅松毛虫雌蛾肠道细菌的物种多样性比雄蛾丰富。雄蛾肠道中丰度差异显著性最高的菌群为α-变形菌纲(Alphaproteobacteria),雌蛾肠道中丰度差异显著性最高的菌群为γ-变形杆菌纲(Gammaproteobacteria)。【结论】思茅松毛虫成虫肠道具有丰富的细菌资源。

【Objective】To study the diversity of gut bacteria from adults of field-collected Dendrolimus kikuchii,which results could lay a foundation for the prevention and control of D. kikuchii and the protection of Pinus yunnanensis and other trees.【Method】The total gut DNA was extracted from male moths and female moths of D. kikuchii respectively.The Illumina NovaSeq(PE250)high-throughput platform was used to sequence and analysis the V3~V4 regions of the gut bacterial 16S rDNA gene,and the gut bacterial community structure and diversity from male adults and female adults of D. kikuchii were analyzed.【Results】A total of 1278 OTUs from adults of D. kikuchii,including 28 phyla,75 classes,173 orders,296 families and 550 genera. The results showed that the dominant bacteria in adults of D. kikuchii gut at the phyla level was Proteobacteria(88.27% for female and 88.17% for male),those at the family level,the dominant bacteria of male moths were Sphingomonadaceae(49.16%)and Comamonadaceae(19.09%),and the dominant bacteria of female moths were Erwiniaceae(34.09%)and Comamonadaceae(16.68%),and those at the genus level,the dominant bacteria of male moths was Sphingomonas(48.09%),and the dominant bacteria of female moths was Pantoea(31.05%). The species diversity of gut bacteria in female moths of D. kikuchii was more abundant than those of male moths. The gut bacteria whose abundance showed the most significant difference was Alphaproteobacteria in male moths of D. kikuchii,and Gammaproteobacteria was in female moths of D. kikuchii.【Conclusion】The species of gut bacteria are rich from the adults of D. kikuchii.