杉木叶片挥发物中对雀丽毒蛾的活性成分鉴定

Identification of Active Volatile Compounds from Cunninghamia lanceolata Leaves to Calliteara melli Collenette

寄主植物挥发性化合物对昆虫定位寄主植物具有重要的影响。为了明确杉木Cunninghamia lanceolata叶片中吸引雀丽毒蛾Calliteara melli的特定挥发性化合物,研究利用GC-MS和GC-EAD分析鉴定了杉木叶片中引起雀丽毒蛾触角电生理反应的挥发物。研究结果表明:杉木叶片中共分离出13种化合物,其中8个为萜类化合物,包括2个半萜化合物,5个倍半萜化合物和1个二萜类化合物,1个烷烃和1个烯烃化合物和3个物质未鉴定化合物。在上述挥发物中,二萜化合物松柏烯的相对含量最高,为48.68%,然后依次为α-蒎烯8.51%、β-瑟林烯7.17%、α-瑟林烯6.09%和β-榄香烯4.28%;其中6种萜类挥发性化合物均能引起雀丽毒蛾雌雄成虫的触角电生理反应,α-蒎烯、β-榄香烯、β-瑟林烯、α-瑟林烯和1-二十四烯均能引起雌蛾和雄蛾的触角反应,β-蒎烯只能引起雌虫的触角反应,对雄蛾没有生理活性。

The volatile compounds of host plants could influence insect orientation to host plants. In order to find out whether there is any volatile compound from Cunninghamia lanceolata leaves that attracts Calliteara melli Collenette in this study, GC-MS and GC-EAD were used to identify the volatiles from the C. lanceolata leave that can elicit the antennal electrophysiological response of the tussock moth. The results showed that 13 compounds were isolated from C. lanceolata leaves, of which 8 were terpenoids, including 2semi-terpenoids, 5 sesquiterpenoids, 1 diterpenoid, 1 alkane, and 1 olefin. In addition, three compounds were not accurately identified. Among all volatiles, the relative content of diterpene was the highest at 48.68%, followed by 8.51% of α-Pinene, 7.17% of β-Silene, 6.09% of α-Silene and 4.28% of β-Elemene. Six terpenoid volatile compounds of α-Pinene, β-Pinene, β-Elemene, β-Silene, α-Silene, and 1-Tetracosene can cause the antennal electrophysiological response of male and female adults. However, β-Pinene can only cause the antennal response of female moths, but not a male adults. These results could provide a scientific reference for the development of host-plant-based attractants for C. melli management.